ArticleBone2014
Fabrication, vascularization and osteogenic properties of a novel synthetic biomimetic induced membrane for the treatment of large bone defects.
Article in Bone, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 55 citations in OpenAlex.
- A review of the Masquelet technique in the treatment of lower limb critical-size bone defects.Annals of the Royal College of Surgeons of England · 2025Review
- Masquelet Inspired in Vivo Engineered Extracellular Matrix as Functional Periosteum for Bone Defect Repair and Reconstruction.Advanced healthcare materials · 2025Article
- Sulfated Polysaccharide Regulates the Homing of HSPCs in a BMP-2-Triggered In Vivo Osteo-Organoid.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- [Application of homemade antibiotic bone cement rod in tibial screw canal osteomyelitis].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2023Article
- Construction of biomimetic cell-sheet-engineered periosteum with a double cell sheet to repair calvarial defects of rats.Journal of orthopaedic translation · 2023Article
- Article
- Construction of developmentally inspired periosteum-like tissue for bone regeneration.Bone research · 2022Article
- [Research progress of tissue engineering technology in promoting revascularization of necrotic femoral bone tissue].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2021Article
- The Application of a Bone Marrow Mesenchymal Stem Cell Membrane in the Vascularization of a Decellularized Tracheal Scaffold.Stem cells international · 2021Article
- [Masquelet technique combined with flap transplantation for infectious bone and soft tissue defects of lower leg].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2020Article
- State-of-the-Art Strategies for the Vascularization of Three-Dimensional Engineered Organs.Vascular specialist international · 2019Review
- Recent advances in cell sheet technology for bone and cartilage regeneration: from preparation to application.International journal of oral science · 2019Review
- [Application of cell sheet technology in bone and cartilage tissue engineering].Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery · 2018Review
- Current data on extremities chronic osteomyelitis in southwest China: epidemiology, microbiology and therapeutic consequences.Scientific reports · 2017Article
- Simulation of ECM with Silk and Chitosan Nanocomposite Materials.Journal of materials chemistry. B · 2017Article
- Article
- The application of cell sheet engineering in the vascularization of tissue regeneration.Regenerative medicine · 2016Review
- Biomaterial-mediated strategies targeting vascularization for bone repair.Drug delivery and translational research · 2016Review
- Silk Biomaterials with Vascularization Capacity.Advanced functional materials · 2016Article
- Induction of granulation tissue for the secretion of growth factors and the promotion of bone defect repair.Journal of orthopaedic surgery and research · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
The induced membrane has been widely used in the treatment of large bone defects but continues to be limited by a relatively lengthy healing process and a requisite two stage surgical procedure. Here we report the development and characterization of a synthetic biomimetic induced membrane (BIM) consisting of an inner highly pre-vascularized cell sheet and an outer osteogenic layer using cell sheet engineering. The pre-vascularized inner layer was formed by seeding human umbilical vein endothelial cells (HUVECs) on a cell sheet comprised of a layer of undifferentiated human bone marrow-derived mesenchymal stem cells (hMSCs). The outer osteogenic layer was formed by inducing osteogenic differentiation of hMSCs. In vitro results indicated that the undifferentiated hMSC cell sheet facilitated the alignment of HUVECs and significantly promoted the formation of vascular-like networks. Furthermore, seeded HUVECs rearranged the extracellular matrix produced by hMSC sheet. After subcutaneous implantation, the composite constructs showed rapid vascularization and anastomosis with the host vascular system, forming functional blood vessels in vivo. Osteogenic potential of the BIM was evidenced by immunohistochemistry staining of osteocalcin, tartrate-resistant acid phosphatase (TRAP) staining, and alizarin red staining. In summary, the synthetic BIM showed rapid vascularization, significant anastomoses, and osteogenic potential in vivo. This synthetic BIM has the potential for treatment of large bone defects in the absence of infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.