ArticlePloS one2014
Production and analysis of perdeuterated lipids from Pichia pastoris cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- Controlled Deuteration of Phosphatidylcholine Biosynthetically Produced from EngineeredACS omega · 2026Article
- Toward Nanodisc Tailoring for SANS Study of Membrane Proteins.Bioengineering (Basel, Switzerland) · 2026Article
- Membrane Charge Drives the Aggregation of TDP-43 Pathological Fragments.Journal of the American Chemical Society · 2025Article
- New Insights into the Interaction of Class II Dihydroorotate Dehydrogenases with Ubiquinone in Lipid Bilayers as a Function of Lipid Composition.International journal of molecular sciences · 2022Article
- Strikingly Different Roles of SARS-CoV-2 Fusion Peptides Uncovered by Neutron Scattering.Journal of the American Chemical Society · 2022Article
- Lipid bilayer degradation induced by SARS-CoV-2 spike protein as revealed by neutron reflectometry.Scientific reports · 2021Article
- Structural Characterization of Natural Yeast Phosphatidylcholine and Bacterial Phosphatidylglycerol Lipid Multilayers by Neutron Diffraction.Frontiers in chemistry · 2021Article
- The Antifungal Mechanism of Amphotericin B Elucidated in Ergosterol and Cholesterol-Containing Membranes Using Neutron Reflectometry.Nanomaterials (Basel, Switzerland) · 2020Article
- About lipid metabolism in Hermetia illucens (L. 1758): on the origin of fatty acids in prepupae.Scientific reports · 2020Article
- Examining Membrane Proteins by Neutron Scattering.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Quantification of Lipids: Model, Reality, and Compromise.Biomolecules · 2018Review
- The effect of hypoxia on the lipidome of recombinant Pichia pastoris.Microbial cell factories · 2017Article
- Mobility of a Mononucleotide within a Lipid Matrix: A Neutron Scattering Study.Life (Basel, Switzerland) · 2017Article
Corrections and comments
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Authors and funding
9 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Probing molecules using perdeuteration (i.e deuteration in which all hydrogen atoms are replaced by deuterium) is extremely useful in a wide range of biophysical techniques. In the case of lipids, the synthesis of the biologically relevant unsaturated perdeuterated lipids is challenging and not usually pursued. In this work, perdeuterated phospholipids and sterols from the yeast Pichia pastoris grown in deuterated medium are extracted and analyzed as derivatives by gas chromatography and mass spectrometry respectively. When yeast cells are grown in a deuterated environment, the phospholipid homeostasis is maintained but the fatty acid unsaturation level is modified while the ergosterol synthesis is not affected by the deuterated culture medium. Our results confirm that the production of well defined natural unsaturated perdeuterated lipids is possible and gives also new insights about the process of desaturase enzymes.
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