ArticleJournal of visualized experiments : JoVE2014
Brain slice biotinylation: an ex vivo approach to measure region-specific plasma membrane protein trafficking in adult neurons.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Enhanced ERK activity extends ketamine's antidepressant effects by augmenting synaptic plasticity.Science (New York, N.Y.) · 2025Article
- Astroglial Kir4.1 potassium channel deficit drives neuronal hyperexcitability and behavioral defects in Fragile X syndrome mouse model.Nature communications · 2024Article
- Cation flux through SUR1-TRPM4 and NCX1 in astrocyte endfeet induces water influx through AQP4 and brain swelling after ischemic stroke.Science signaling · 2023Article
- Spinophilin-dependent regulation of GluN2B-containing NMDAR-dependent calcium influx, GluN2B surface expression, and cleaved caspase expression.Synapse (New York, N.Y.) · 2023Article
- Presynaptic Gq-coupled receptors drive biphasic dopamine transporter trafficking that modulates dopamine clearance and motor function.The Journal of biological chemistry · 2023Article
- Molecular and structural basis of olfactory sensory neuron axon coalescence by Kirrel receptors.Cell reports · 2021Article
- Early-life stress alters affective behaviors in adult mice through persistent activation of CRH-BDNF signaling in the oval bed nucleus of the stria terminalis.Translational psychiatry · 2020Article
- Neuronal Activity Drives Astroglial Connexin 30 in Perisynaptic Processes and Shapes Its Functions.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Chronic Stress Induces Maladaptive Behaviors by Activating Corticotropin-Releasing Hormone Signaling in the Mouse Oval Bed Nucleus of the Stria Terminalis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Disentangling Biomolecular Corona Interactions With Cell Receptors and Implications for Targeting of Nanomedicines.Frontiers in bioengineering and biotechnology · 2020Review
- Cdk5 Contributes to Huntington's Disease Learning and Memory Deficits via Modulation of Brain Region-Specific Substrates.Molecular neurobiology · 2018Article
- Region-Specific Regulation of Presynaptic Dopamine Homeostasis by DThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Cereblon Maintains Synaptic and Cognitive Function by Regulating BK Channel.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method.Journal of visualized experiments : JoVE · 2018Article
- Molecular Dissection of Neuroligin 2 and Slitrk3 Reveals an Essential Framework for GABAergic Synapse Development.Neuron · 2017Article
- Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake.Journal of visualized experiments : JoVE · 2017Article
- Ack1 is a dopamine transporter endocytic brake that rescues a trafficking-dysregulated ADHD coding variant.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- An effective plasma membrane proteomics approach for small tissue samples.Scientific reports · 2015Article
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3 authors.
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Abstract
Regulated endocytic trafficking is the central mechanism facilitating a variety of neuromodulatory events, by dynamically controlling receptor, ion channel, and transporter cell surface presentation on a minutes time scale. There is a broad diversity of mechanisms that control endocytic trafficking of individual proteins. Studies investigating the molecular underpinnings of trafficking have primarily relied upon surface biotinylation to quantitatively measure changes in membrane protein surface expression in response to exogenous stimuli and gene manipulation. However, this approach has been mainly limited to cultured cells, which may not faithfully reflect the physiologically relevant mechanisms at play in adult neurons. Moreover, cultured cell approaches may underestimate region-specific differences in trafficking mechanisms. Here, we describe an approach that extends cell surface biotinylation to the acute brain slice preparation. We demonstrate that this method provides a high-fidelity approach to measure rapid changes in membrane protein surface levels in adult neurons. This approach is likely to have broad utility in the field of neuronal endocytic trafficking.
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