ArticlePloS one2014
A simple and effective method for construction of Escherichia coli strains proficient for genome engineering.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Microbial Cell Factory for Efficiently Synthesizing Plant Natural Products via Optimizing the Location and Adaptation of Pathway on Genome Scale.Frontiers in bioengineering and biotechnology · 2020Review
- A 'Semi-Protected Oligonucleotide Recombination' Assay for DNA Mismatch Repair in vivo Suggests Different Modes of Repair for Lagging Strand Mismatches.Nucleic acids research · 2017Article
- Oligo- and dsDNA-mediated genome editing using a tetA dual selection system in Escherichia coli.PloS one · 2017Article
- Recent advances and versatility of MAGE towards industrial applications.Systems and synthetic biology · 2015Review
- Article
- Differential requirements of singleplex and multiplex recombineering of large DNA constructs.PloS one · 2015Article
- Article
- Genome engineering for improved recombinant protein expression in Escherichia coli.Microbial cell factories · 2014Review
- Modern and simple construction of plasmid: saving time and cost.Journal of microbiology (Seoul, Korea) · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multiplex genome engineering is a standalone recombineering tool for large-scale programming and accelerated evolution of cells. However, this advanced genome engineering technique has been limited to use in selected bacterial strains. We developed a simple and effective strain-independent method for effective genome engineering in Escherichia coli. The method involves introducing a suicide plasmid carrying the λ Red recombination system into the mutS gene. The suicide plasmid can be excised from the chromosome via selection in the absence of antibiotics, thus allowing transient inactivation of the mismatch repair system during genome engineering. In addition, we developed another suicide plasmid that enables integration of large DNA fragments into the lacZ genomic locus. These features enable this system to be applied in the exploitation of the benefits of genome engineering in synthetic biology, as well as the metabolic engineering of different strains of E. coli.
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Registered trials
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