ArticleEukaryotic cell2014
Cryptococcus neoformans dual GDP-mannose transporters and their role in biology and virulence.
Article in Eukaryotic cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Structural basis for transport and inhibition of nucleotide sugar transport in pathogenic fungi.Nature communications · 2026Article
- Article
- Virulence factors released by extracellular vesicles fromFrontiers in cellular and infection microbiology · 2025Review
- Article
- Illuminating the Cryptococcus neoformans species complex: unveiling intracellular structures with fluorescent-protein-based markers.Genetics · 2024Article
- Expansion of healthcare-associated hypervirulent KPC-2-producingOne health (Amsterdam, Netherlands) · 2023Article
- Unbiased discovery of natural sequence variants that influence fungal virulence.Cell host & microbe · 2023Article
- Article
- Biology and function of exo-polysaccharides from human fungal pathogens.Current clinical microbiology reports · 2020Article
- Review
- Unraveling synthesis of the cryptococcal cell wall and capsule.Glycobiology · 2018Review
- Article
- Peeling the onion: the outer layers of Cryptococcus neoformans.Memorias do Instituto Oswaldo Cruz · 2018Review
- Xylose donor transport is critical for fungal virulence.PLoS pathogens · 2018Article
- Nutritional Requirements and Their Importance for Virulence of Pathogenic Cryptococcus Species.Microorganisms · 2017Review
- Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter.Glycobiology · 2017Article
- Virulence Factors as Targets for Anticryptococcal Therapy.Journal of fungi (Basel, Switzerland) · 2016Review
- Article
- Model-driven mapping of transcriptional networks reveals the circuitry and dynamics of virulence regulation.Genome research · 2015Article
- Cryptococcus neoformans Yap1 is required for normal fluconazole and oxidative stress resistance.Fungal genetics and biology : FG & B · 2015Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Cryptococcus neoformans is an opportunistic yeast responsible for lethal meningoencephalitis in humans. This pathogen elaborates a polysaccharide capsule, which is its major virulence factor. Mannose constitutes over one-half of the capsule mass and is also extensively utilized in cell wall synthesis and in glycosylation of proteins and lipids. The activated mannose donor for most biosynthetic reactions, GDP-mannose, is made in the cytosol, although it is primarily consumed in secretory organelles. This compartmentalization necessitates specific transmembrane transporters to make the donor available for glycan synthesis. We previously identified two cryptococcal GDP-mannose transporters, Gmt1 and Gmt2. Biochemical studies of each protein expressed in Saccharomyces cerevisiae showed that both are functional, with similar kinetics and substrate specificities in vitro. We have now examined these proteins in vivo and demonstrate that cells lacking Gmt1 show significant phenotypic differences from those lacking Gmt2 in terms of growth, colony morphology, protein glycosylation, and capsule phenotypes. Some of these observations may be explained by differential expression of the two genes, but others suggest that the two proteins play overlapping but nonidentical roles in cryptococcal biology. Furthermore, gmt1 gmt2 double mutant cells, which are unexpectedly viable, exhibit severe defects in capsule synthesis and protein glycosylation and are avirulent in mouse models of cryptococcosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.