ArticleJournal of visualized experiments : JoVE2014
The ChroP approach combines ChIP and mass spectrometry to dissect locus-specific proteomic landscapes of chromatin.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Current Analytical Strategies in Studying Chromatin-Associated-Proteome (Chromatome).Molecules (Basel, Switzerland) · 2021Review
- A distinct isoform of ZNF207 controls self-renewal and pluripotency of human embryonic stem cells.Nature communications · 2018Article
- Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae.Journal of visualized experiments : JoVE · 2017Article
- The histone variant H3.3 G34W substitution in giant cell tumor of the bone link chromatin and RNA processing.Scientific reports · 2017Article
- Unravelling the biology of chromatin in health and cancer using proteomic approaches.Expert review of proteomics · 2017Review
- Mass Spectrometry-Based Tools to Characterize DNA-Protein Cross-Linking by Bis-Electrophiles.Basic & clinical pharmacology & toxicology · 2017Review
- SILAC Based Proteomic Characterization of Exosomes from HIV-1 Infected Cells.Journal of visualized experiments : JoVE · 2017Article
- Photosensitized UVA-Induced Cross-Linking between Human DNA Repair and Replication Proteins and DNA Revealed by Proteomic Analysis.Journal of proteome research · 2016Article
- Unlocking the chromatin code by deciphering protein-DNA interactions.Molecular systems biology · 2016Article
- Proteomics to study DNA-bound and chromatin-associated gene regulatory complexes.Human molecular genetics · 2016Article
- Rapid immunoprecipitation mass spectrometry of endogenous proteins (RIME) for analysis of chromatin complexes.Nature protocols · 2016Article
- Chromatin proteomic profiling reveals novel proteins associated with histone-marked genomic regions.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Progress in epigenetic histone modification analysis by mass spectrometry for clinical investigations.Expert review of proteomics · 2015Review
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2 authors at 1 institution in 1 country.
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Abstract
Chromatin is a highly dynamic nucleoprotein complex made of DNA and proteins that controls various DNA-dependent processes. Chromatin structure and function at specific regions is regulated by the local enrichment of histone post-translational modifications (hPTMs) and variants, chromatin-binding proteins, including transcription factors, and DNA methylation. The proteomic characterization of chromatin composition at distinct functional regions has been so far hampered by the lack of efficient protocols to enrich such domains at the appropriate purity and amount for the subsequent in-depth analysis by Mass Spectrometry (MS). We describe here a newly designed chromatin proteomics strategy, named ChroP (Chromatin Proteomics), whereby a preparative chromatin immunoprecipitation is used to isolate distinct chromatin regions whose features, in terms of hPTMs, variants and co-associated non-histonic proteins, are analyzed by MS. We illustrate here the setting up of ChroP for the enrichment and analysis of transcriptionally silent heterochromatic regions, marked by the presence of tri-methylation of lysine 9 on histone H3. The results achieved demonstrate the potential of ChroP in thoroughly characterizing the heterochromatin proteome and prove it as a powerful analytical strategy for understanding how the distinct protein determinants of chromatin interact and synergize to establish locus-specific structural and functional configurations.
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