Evidence map›Paper›PMID 24746557›Full record

ArticleContraception2014

Does tenofovir gel or do other microbicide products affect detection of biomarkers of semen exposure in vitro?

Margaret C Snead, Athena P Kourtis, Johan H Melendez, Carolyn M Black, Christine K Mauck, Ana Penman-Aguilar, Dorothy M Chaney, Maria F Gallo, Denise J Jamieson, Maurizio Macaluso and 1 more

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Contraception, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Observational
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 7 institutions in 1 country.

Margaret C SneadThe Division of Reproductive Health, National Center for Chronic Disease Prevention and Health Promotion, Baltimore, MD, USA. Electronic address: msnead@cdc.gov.
Athena P KourtisThe Division of Reproductive Health, National Center for Chronic Disease Prevention and Health Promotion, Baltimore, MD, USA.
Johan H MelendezJohns Hopkins University School of Medicine, Baltimore, MD, USA.
Carolyn M BlackThe Division of Scientific Resources, National Center for Emerging and Ζοοnotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
Christine K MauckCONRAD, Eastern Virginia Medical School (EVMS), Arlington, VA, USA.
Ana Penman-AguilarThe Division of Reproductive Health, National Center for Chronic Disease Prevention and Health Promotion, Baltimore, MD, USA.
Dorothy M ChaneyJohns Hopkins University School of Medicine, Baltimore, MD, USA.
Maria F GalloThe Division of Reproductive Health, National Center for Chronic Disease Prevention and Health Promotion, Baltimore, MD, USA.
Denise J JamiesonThe Division of Reproductive Health, National Center for Chronic Disease Prevention and Health Promotion, Baltimore, MD, USA.
Maurizio MacalusoDivision of Biostatistics and Epidemiology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH, USA.
Gustavo F DoncelCONRAD, Eastern Virginia Medical School (EVMS), Arlington, VA, USA.
National Center for Chronic Disease Prevention and Health Promotion · USCincinnati Children's Hospital Medical Center · USContraception Research and Development · USEastern Virginia Medical School · USJohns Hopkins Medicine · USJohns Hopkins University · USNational Center for Emerging and Zoonotic Infectious Diseases · US

Funding

Intramural CDC HHS CC999999
6 · The paper itself

Abstract

objectivesThere is currently no information on whether products evaluated in HIV microbicide trials affect the detection of the semen biomarkers prostate-specific antigen (PSA) or Y chromosome DNA. STUDY

designWe tested (in vitro) dilutions of tenofovir (TFV), UC781 and the hydroxyethylcellulose (HEC) placebo gels using the Abacus ABAcard and the quantitative (Abbott Architect total PSA) assays for PSA and Y chromosome DNA by real-time polymerase chain reaction.

resultsTFV gel and the HEC placebo adversely affected PSA detection using the ABAcard but not the Abbott Architect total PSA assay. UC781 adversely affected both the ABAcard and Abbott Architect total PSA assays. While there were some quantitative changes in the magnitude of the signal, none of the products affected positivity of the Y chromosome assay.

conclusionsThe presence of TFV or HEC gels did not affect quantitative PSA or Y chromosome detection in vitro. Confirmation of these findings is recommended using specimens obtained following use of these gels in vivo. IMPLICATIONS: Researchers should consider the potential for specific microbicides or any products to affect the particular assay used for semen biomarker detection. The ABAcard assay for PSA detection should not be used with TFV UC781, or HEC.

Indexed as

AdenineAnilidesAntibiotic ProphylaxisBiomarkersCelluloseChromosomes, Human, YDNAExcipientsFalse Positive ReactionsFemaleFuransHumansLimit of DetectionOrganophosphonatesOsmolar ConcentrationProstate-Specific AntigenAdenineAnilidesBiomarkersCelluloseDNAExcipientsFuranshydroxyethylcelluloseOrganophosphonatesProstate-Specific AntigenReverse Transcriptase InhibitorsTenofovirThioamidesUC-781Vaginal Creams, Foams, and JelliesBiomarkersGelsHEC placeboHydroxyethylcelluloseMicrobicidesProstate-specific antigenSemenTenofovirUC781Y chromosome

Identifiers

PMID24746557
PMCPMC4547342
OpenAlexW2029204408

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.