ArticleJournal of the American Society of Nephrology : JASN2014
Aldosterone regulates microRNAs in the cortical collecting duct to alter sodium transport.
Article in Journal of the American Society of Nephrology : JASN, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 30 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 48 citations in OpenAlex.
- ENaC phosphorylation facilitates ankyrin-3 interaction critical for renal sodium balance.PNAS nexus · 2026Article
- Epigenetic Regulation of the Renin-Angiotensin-Aldosterone System in Hypertension.International journal of molecular sciences · 2024Review
- MicroRNA-19 is regulated by aldosterone in a sex-specific manner to alter kidney sodium transport.American journal of physiology. Cell physiology · 2024Article
- An in vitro approach to understand contribution of kidney cells to human urinary extracellular vesicles.Journal of extracellular vesicles · 2023Article
- Homozygous Missense Variant in the N-Terminal Region of ANK3 Gene Is Associated with Developmental Delay, Seizures, Speech Abnormality, and Aggressive Behavior.Molecular syndromology · 2023Article
- miR-324-5p and miR-30c-2-3p Alter Renal Mineralocorticoid Receptor Signaling under Hypertonicity.Cells · 2022Article
- Hypothalamic miR-1983 Targets Insulin Receptor β and the Insulin-mediated miR-1983 Increase Is Blocked by Metformin.Endocrinology · 2022Article
- Mechanisms and consequences of casein kinase II and ankyrin-3 regulation of the epithelial NaScientific reports · 2021Article
- Non-coding RNAs and the mineralocorticoid receptor in the kidney.Molecular and cellular endocrinology · 2021Review
- Aldosterone-induced microRNAs act as feedback regulators of mineralocorticoid receptor signaling in kidney epithelia.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020Article
- Kras mutations and PU.1 promoter methylation are new pathways in murine radiation-induced AML.Carcinogenesis · 2020Article
- Prostaglandin E2 stimulates the epithelial sodium channel (ENaC) in cultured mouse cortical collecting duct cells in an autocrine manner.The Journal of general physiology · 2020Article
- The Role of MicroRNA in the Airway Surface Liquid Homeostasis.International journal of molecular sciences · 2020Review
- Upregulation of the Coatomer Protein Complex Subunit beta 2 (COPB2) Gene Targets microRNA-335-3p in NCI-H1975 Lung Adenocarcinoma Cells to Promote Cell Proliferation and Migration.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- Lipopolysaccharide Inhibits Alpha Epithelial Sodium Channel Expression via MiR-124-5p in Alveolar Type 2 Epithelial Cells.BioMed research international · 2020Article
- Loss ofAmerican journal of physiology. Renal physiology · 2019Article
- miR-137 Affects Vaginal Lubrication in Female Sexual Dysfunction by Targeting Aquaporin-2.Sexual medicine · 2018Article
- miR-34c-5p and CaMKII are involved in aldosterone-induced fibrosis in kidney collecting duct cells.American journal of physiology. Renal physiology · 2018Article
- Physiological regulation of the epithelial NaAmerican journal of physiology. Renal physiology · 2018Article
- Dysregulated miRNAs and their pathogenic implications for the neurometabolic disease propionic acidemia.Scientific reports · 2017Article
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Authors and funding
10 authors at 1 institution in 2 countries.
Funding
Abstract
A role for microRNAs (miRs) in the physiologic regulation of sodium transport in the kidney has not been established. In this study, we investigated the potential of aldosterone to alter miR expression in mouse cortical collecting duct (mCCD) epithelial cells. Microarray studies demonstrated the regulation of miR expression by aldosterone in both cultured mCCD and isolated primary distal nephron principal cells. Aldosterone regulation of the most significantly downregulated miRs, mmu-miR-335-3p, mmu-miR-290-5p, and mmu-miR-1983 was confirmed by quantitative RT-PCR. Reducing the expression of these miRs separately or in combination increased epithelial sodium channel (ENaC)-mediated sodium transport in mCCD cells, without mineralocorticoid supplementation. Artificially increasing the expression of these miRs by transfection with plasmid precursors or miR mimic constructs blunted aldosterone stimulation of ENaC transport. Using a newly developed computational approach, termed ComiR, we predicted potential gene targets for the aldosterone-regulated miRs and confirmed ankyrin 3 (Ank3) as a novel aldosterone and miR-regulated protein. A dual-luciferase assay demonstrated direct binding of the miRs with the Ank3-3' untranslated region. Overexpression of Ank3 increased and depletion of Ank3 decreased ENaC-mediated sodium transport in mCCD cells. These findings implicate miRs as intermediaries in aldosterone signaling in principal cells of the distal kidney nephron.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.