ArticleNucleic acids research2014
Eukaryotic and archaeal TBP and TFB/TF(II)B follow different promoter DNA bending pathways.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- Single-molecule dynamic structural biology with vertically arranged DNA on a fluorescence microscope.Nature methods · 2025Article
- Structural basis of archaeal RNA polymerase transcription elongation and Spt4/5 recruitment.Nucleic acids research · 2024Article
- Cbp1 and Cren7 form chromatin-like structures that ensure efficient transcription of long CRISPR arrays.Nature communications · 2024Article
- Promoter-proximal elongation regulates transcription in archaea.Nature communications · 2021Article
- DNA origami-based single-molecule force spectroscopy elucidates RNA Polymerase III pre-initiation complex stability.Nature communications · 2020Article
- The Role of Archaeal Chromatin in Transcription.Journal of molecular biology · 2019Review
- The cutting edge of archaeal transcription.Emerging topics in life sciences · 2018Article
- Displacement of the transcription factor B reader domain during transcription initiation.Nucleic acids research · 2018Article
- A transcriptional factor B paralog functions as an activator to DNA damage-responsive expression in archaea.Nucleic acids research · 2018Article
- The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor.Nature communications · 2017Article
- Molecular mechanisms of Bdp1 in TFIIIB assembly and RNA polymerase III transcription initiation.Nature communications · 2017Article
- Same same but different: The evolution of TBP in archaea and their eukaryotic offspring.Transcription · 2017Article
- A global analysis of transcription reveals two modes of Spt4/5 recruitment to archaeal RNA polymerase.Nature microbiology · 2017Article
- Repression of RNA polymerase by the archaeo-viral regulator ORF145/RIP.Nature communications · 2016Article
- Molecular force spectroscopy with a DNA origami-based nanoscopic force clamp.Science (New York, N.Y.) · 2016Article
- Transcription Regulation in Archaea.Journal of bacteriology · 2016Review
- Molecular Mechanisms of Transcription Initiation-Structure, Function, and Evolution of TFE/TFIIE-Like Factors and Open Complex Formation.Journal of molecular biology · 2016Review
- Structural and functional insights into the fly microRNA biogenesis factor Loquacious.RNA (New York, N.Y.) · 2016Article
- A continuum model of transcriptional bursting.eLife · 2016Article
- Super-Resolution Imaging Conditions for enhanced Yellow Fluorescent Protein (eYFP) Demonstrated on DNA Origami Nanorulers.Scientific reports · 2015Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
During transcription initiation, the promoter DNA is recognized and bent by the basal transcription factor TATA-binding protein (TBP). Subsequent association of transcription factor B (TFB) with the TBP-DNA complex is followed by the recruitment of the ribonucleic acid polymerase resulting in the formation of the pre-initiation complex. TBP and TFB/TF(II)B are highly conserved in structure and function among the eukaryotic-archaeal domain but intriguingly have to operate under vastly different conditions. Employing single-pair fluorescence resonance energy transfer, we monitored DNA bending by eukaryotic and archaeal TBPs in the absence and presence of TFB in real-time. We observed that the lifetime of the TBP-DNA interaction differs significantly between the archaeal and eukaryotic system. We show that the eukaryotic DNA-TBP interaction is characterized by a linear, stepwise bending mechanism with an intermediate state distinguished by a distinct bending angle. TF(II)B specifically stabilizes the fully bent TBP-promoter DNA complex and we identify this step as a regulatory checkpoint. In contrast, the archaeal TBP-DNA interaction is extremely dynamic and TBP from the archaeal organism Sulfolobus acidocaldarius strictly requires TFB for DNA bending. Thus, we demonstrate that transcription initiation follows diverse pathways on the way to the formation of the pre-initiation complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.