Evidence map›Paper›PMID 24743600›Full record

ArticlePloS one2014

Genetic control of differential acetylation in diabetic rats.

Pamela J Kaisaki, Georg W Otto, Joanna F McGouran, Amine Toubal, Karène Argoud, Helen Waller-Evans, Clare Finlay, Sophie Caldérari, Marie-Thérèse Bihoreau, Benedikt M Kessler and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Pamela J KaisakiThe Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United Kingdom.
Georg W OttoThe Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United Kingdom.
Joanna F McGouranTarget Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Amine ToubalINSERM, U872, Cordeliers Research Centre, Paris, France; Institute of Cardiometabolism & Nutrition, ICAN, Pitié-Salpêtrière Hospital, University Pierre & Marie-Curie, Paris, France.
Karène ArgoudThe Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United Kingdom.
Helen Waller-EvansThe Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United Kingdom.
Clare FinlayThe Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United Kingdom.
Sophie CaldérariINSERM, U872, Cordeliers Research Centre, Paris, France; Institute of Cardiometabolism & Nutrition, ICAN, Pitié-Salpêtrière Hospital, University Pierre & Marie-Curie, Paris, France.
Marie-Thérèse BihoreauNational Genotyping Centre, Evry, France.
Benedikt M KesslerTarget Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom.
Dominique GauguierINSERM, U872, Cordeliers Research Centre, Paris, France; Institute of Cardiometabolism & Nutrition, ICAN, Pitié-Salpêtrière Hospital, University Pierre & Marie-Curie, Paris, France.
Richard MottThe Wellcome Trust Centre for Human Genetics, University of Oxford, Headington, Oxford, United Kingdom.
University of Oxford · GBCentre for Human Genetics · GBCentre de Recherche des Cordeliers · FRCentre National de Recherche en Génomique Humaine · FRUnité de recherche sur les maladies cardiovasculaires et métaboliques · FR

Funding

Medical Research Council G0900747Medical Research Council G0900747 91070Wellcome TrustWellcome Trust 057733Wellcome Trust 075491/Z/04Wellcome Trust 083573/Z/07/ZWellcome Trust 090532/Z/09/Z
6 · The paper itself

Abstract

Post-translational protein modifications such as acetylation have significant regulatory roles in metabolic processes, but their relationship to both variation in gene expression and DNA sequence is unclear. We address this question in the Goto-Kakizaki (GK) rat inbred strain, a model of polygenic type 2 diabetes. Expression of the NAD-dependent deacetylase Sirtuin-3 is down-regulated in GK rats compared to normoglycemic Brown Norway (BN) rats. We show first that a promoter SNP causes down-regulation of Sirtuin-3 expression in GK rats. We then use mass-spectrometry to identify proteome-wide differential lysine acetylation of putative Sirtuin-3 protein targets in livers of GK and BN rats. These include many proteins in pathways connected to diabetes and metabolic syndrome. We finally sequence GK and BN liver transcriptomes and find that mRNA expression of these targets does not differ significantly between GK and BN rats, in contrast to other components of the same pathways. We conclude that physiological differences between GK and BN rats are mediated by a combination of differential protein acetylation and gene transcription and that genetic variation can modulate acetylation independently of expression.

Indexed as

AcetylationAcetyltransferasesAmino AcidsAnimalsCitric Acid CycleDiabetes Mellitus, ExperimentalDiabetes Mellitus, Type 2Fatty AcidsGene Expression RegulationGluconeogenesisGlycolysisLiverMalePentose Phosphate PathwayPolymorphism, GeneticProtein Processing, Post-TranslationalAcetyltransferasesAmino AcidsFatty AcidspurinePurinespyrimidinePyrimidinesSirtuin 3

Identifiers

PMID24743600
PMCPMC3990556
OpenAlexW2049296646

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.