ArticlePloS one2014
Development of a chemically defined medium and discovery of new mitogenic growth factors for mouse hepatocytes: mitogenic effects of FGF1/2 and PDGF.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- A Novel Role for the Phosphatidylinositol-4,5-Bisphosphate 3-Kinase Delta Isoform in Hepatocellular Proliferation.The American journal of pathology · 2024Article
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- FoxO3 restricts liver regeneration by suppressing the proliferation of hepatocytes.NPJ Regenerative medicine · 2022Article
- Photopatterned biomolecule immobilization to guide three-dimensional cell fate in natural protein-based hydrogels.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A simple method for isolation and culture of primary hepatocytes from Salvelinus leucomaenis (White-spotted Charr).Cytotechnology · 2020Article
- Article
- Optimization of DamID for use in primary cultures of mouse hepatocytes.Methods (San Diego, Calif.) · 2019Article
- The Polyploid State Restricts Hepatocyte Proliferation and Liver Regeneration in Mice.Hepatology (Baltimore, Md.) · 2019Article
- Novel sericin-based hepatocyte serum-free medium and sericin's effect on hepatocyte transcriptome.World journal of gastroenterology · 2018Article
- A polyelectrolyte multilayer platform for investigating growth factor delivery modes in human liver cultures.Journal of biomedical materials research. Part A · 2018Article
- Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes.EBioMedicine · 2017Article
- Arsenite Effects on Mitochondrial Bioenergetics in Human and Mouse Primary Hepatocytes Follow a Nonlinear Dose Response.Oxidative medicine and cellular longevity · 2017Article
- Integration of novel approaches demonstrates simultaneous metabolic inactivation and CAR-mediated hepatocarcinogenesis of a nitrification inhibitor.Toxicology reports · 2017Article
- Regulation of Hepatic Stellate Cells and Fibrogenesis by Fibroblast Growth Factors.BioMed research international · 2016Review
- PDGFRα in liver pathophysiology: emerging roles in development, regeneration, fibrosis, and cancer.Gene expression · 2015Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Chemically defined serum-free media for rat hepatocytes have been useful in identifying EGFR ligands and HGF/MET signaling as direct mitogenic factors for rat hepatocytes. The absence of such media for mouse hepatocytes has prevented screening for discovery of such mitogens for mouse hepatocytes. We present results obtained by designing such a chemically defined medium for mouse hepatocytes and demonstrate that in addition to EGFR ligands and HGF, the growth factors FGF1 and FGF2 are also important mitogenic factors for mouse hepatocytes. Smaller mitogenic response was also noticed for PDGF AB. Mouse hepatocytes are more likely to enter into spontaneous proliferation in primary culture due to activation of cell cycle pathways resulting from collagenase perfusion. These results demonstrate unanticipated fundamental differences in growth biology of hepatocytes between the two rodent species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.