ArticleNature protocols2014
Synthesis, labeling and bioanalytical applications of a tris(2,2'-bipyridyl)ruthenium(II)-based electrochemiluminescence probe.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Interface Self-Assembly of AuSmall (Weinheim an der Bergstrasse, Germany) · 2026Article
- An Experimental and Molecular modelling Study of Ruthenium(II) Polypyridyl Complexes with a Thiazole-Imidazo-Phenanthroline Ligand and their DNA Affinity.Journal of fluorescence · 2026Article
- Ruthenium Complexes in Protein Science: Heading a New Generation of Therapeutic and Analytical Tools.Angewandte Chemie (International ed. in English) · 2026Review
- Bright Circularly Polarized Electrochemiluminescence from Heterobinuclear IrAngewandte Chemie (International ed. in English) · 2026Article
- Development of electrochemiluminescence immunochromatographic test strips for dengue virus.Frontiers in bioengineering and biotechnology · 2026Article
- Development of paper-based electrochemiluminescence test strips for pathogenic microorganisms.BMC infectious diseases · 2025Article
- From theory to practice: understanding the challenges in the implementation of electrogenerated chemiluminescence for analytical applications.Mikrochimica acta · 2024Review
- An Alternating Current Electroosmotic Flow-Based Ultrasensitive Electrochemiluminescence Microfluidic System for Ultrafast Monitoring, Detection of Proteins/miRNAs in Unprocessed Samples.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Redox-mediated electrochemiluminescence enhancement for bead-based immunoassay.Chemical science · 2024Article
- Rapid and sensitive electrochemiluminescence detection using easily fabricated sensor with an integrated two-electrode system.RSC advances · 2024Article
- Tris(2,2'-bipyridyl)ruthenium (II) complex as a universal reagent for the fabrication of heterogeneous electrochemiluminescence platforms and its recent analytical applications.Analytical and bioanalytical chemistry · 2023Review
- Ubiquitination detection techniques.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- A Study of the Detection of SARS-CoV-2 by the Use of Electrochemiluminescent Biosensor Based on Asymmetric Polymerase Chain Reaction Amplification Strategy.IEEE sensors journal · 2023Article
- Nucleotide detection mechanism and comparison based on low-dimensional materials: A review.Frontiers in bioengineering and biotechnology · 2023Review
- Portable paper-based electrochemiluminescence test incorporating lateral-flow immunosensors for detection of interferon-γ levels.Frontiers in bioengineering and biotechnology · 2023Article
- Studies on Annihilation and Coreactant Electrochemiluminescence of Thermally Activated Delayed Fluorescent Molecules in Organic Medium.Molecules (Basel, Switzerland) · 2022Article
- Recent advances in the potential applications of luminescence-based, SPR-based, and carbon-based biosensors.Applied microbiology and biotechnology · 2022Review
- Electrochemiluminescent immunoassay for the determination of CA15-3 and CA72-4 using graphene oxide nanocomposite modified with CdSe quantum dots and Ru(bpy)Mikrochimica acta · 2021Article
- Spooling electrochemiluminescence spectroscopy: development, applications and beyond.Nature protocols · 2021Article
- CRISPR/Cas13a Powered Portable Electrochemiluminescence Chip for Ultrasensitive and Specific MiRNA Detection.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Assays using probes labeled with electrochemiluminescent moieties are extremely powerful analytical tools that are used in fields such as medical diagnostics, environmental analysis and food safety monitoring, in which sensitive, reliable and reproducible detection of biomolecules is a requirement. The most efficient electrochemiluminescence (ECL) reaction to date is based on tris(2,2'-bipyridyl)ruthenium(II) (Ru(bpy)3(2+)) with tripropylamine (TPrA) as the co-reactant. Here we present a detailed protocol for preparing Ru(bpy)3(2+) probes and their bioanalytical applications. This protocol includes (i) the synthesis of a biologically active Ru(bpy)3(2+)-N-hydroxysuccinimide (NHS) ester, (ii) its covalent labeling with both antibodies and DNA probes and (iii) the detection and quantification of ECL in a microfluidic system with a paramagnetic microbead solid support. In our magnetic bead-based ECL system, two probes are required: a capture probe (labeled with biotin to be captured by a streptavidin-coated magnetic bead) and a detector probe (labeled with Ru(bpy)3(2+)). The complex consisting of the analyte, the capture probe, the detector probe and the magnetic bead is brought into contact with the electrode by using a magnetic field. The Ru(bpy)3(2+) reacts with TPrA in solution to generate the ECL signal. The full protocol, including the synthesis and labeling of the bioactive Ru(bpy)3(2+), requires 5-6 d to complete. ECL immunoassays or nucleic acid tests only require 1.5-2 h, including the sample preparation time.
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