ArticleNature protocols2014
Multiparametric high-resolution imaging of native proteins by force-distance curve-based AFM.
Article in Nature protocols, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Control of a Gel-Forming Chemical Reaction Network Using Light-Triggered Proton Pumps.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Effects of serum proteins on corrosion rates and product bioabsorbability of biodegradable metals.Regenerative biomaterials · 2024Article
- The insertase YidC chaperones the polytopic membrane protein MelB inserting and folding simultaneously from both termini.Structure (London, England : 1993) · 2023Article
- Substrate-binding guides individual melibiose permeases MelB to structurally soften and to destabilize cytoplasmic middle-loop C3.Structure (London, England : 1993) · 2023Article
- Covalent organic framework membranes for efficient separation of monovalent cations.Nature communications · 2022Article
- Antibiotic polymyxin arranges lipopolysaccharide into crystalline structures to solidify the bacterial membrane.Nature communications · 2022Article
- Supramolecular systems chemistry through advanced analytical techniques.Analytical and bioanalytical chemistry · 2022Review
- A Critical Review on the Sensing, Control, and Manipulation of Single Molecules on Optofluidic Devices.Micromachines · 2022Review
- Gasdermin-A3 pore formation propagates along variable pathways.Nature communications · 2022Article
- Investigation of HIV-1 Gag binding with RNAs and lipids using Atomic Force Microscopy.PloS one · 2020Article
- Mechanical Characterization of Liposomes and Extracellular Vesicles, a Protocol.Frontiers in molecular biosciences · 2020Article
- Solidifying framework nucleic acids with silica.Nature protocols · 2019Article
- Differentiating between Inactive and Active States of Rhodopsin by Atomic Force Microscopy in Native Membranes.Analytical chemistry · 2019Article
- Seeing and sensing single G protein-coupled receptors by atomic force microscopy.Current opinion in cell biology · 2019Review
- Fast and high-resolution mapping of elastic properties of biomolecules and polymers with bimodal AFM.Nature protocols · 2018Article
- Microfluidic deposition for resolving single-molecule protein architecture and heterogeneity.Nature communications · 2018Article
- Printing two-dimensional gallium phosphate out of liquid metal.Nature communications · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A current challenge in the life sciences is to understand how the properties of individual molecular machines adjust in order to meet the functional requirements of the cell. Recent developments in force-distance (FD) curve-based atomic force microscopy (FD-based AFM) enable researchers to combine sub-nanometer imaging with quantitative mapping of physical, chemical and biological properties. Here we present a protocol to apply FD-based AFM to the multiparametric imaging of native proteins under physiological conditions. We describe procedures for experimental FD-based AFM setup, high-resolution imaging of proteins in the native unperturbed state with simultaneous quantitative mapping of multiple parameters, and data interpretation and analysis. The protocol, which can be completed in 1-3 d, enables researchers to image proteins and protein complexes in the native unperturbed state and to simultaneously map their biophysical and biochemical properties at sub-nanometer resolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.