ArticlePhysics in medicine and biology2014
Accounting for pharmacokinetic differences in dual-tracer receptor density imaging.
Article in Physics in medicine and biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 29 citations in OpenAlex.
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- Prediction of optimal contrast times post-imaging agent administration to inform personalized fluorescence-guided surgery.Journal of biomedical optics · 2020Article
- Intracellular paired agent imaging enables improved evaluation of tyrosine kinase inhibitor target engagement.Proceedings of SPIE--the International Society for Optical Engineering · 2020Article
- Paired-agent imaging for detection of head and neck cancers.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Noninvasive imaging of dual-agent uptake in glioma and normal tissue using MRI-coupled fluorescence tomography.Proceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Simultaneous extracellular and intracellular quantification of EGFR using paired-agent imaging in anProceedings of SPIE--the International Society for Optical Engineering · 2019Article
- Perspective review of what is needed for molecular-specific fluorescence-guided surgery.Journal of biomedical optics · 2018Review
- Correcting for targeted and control agent signal differences in paired-agent molecular imaging of cancer cell-surface receptors.Journal of biomedical optics · 2018Article
- 3D Imaging Detection of HER2 Based in the Use of Novel Affibody-Quantum Dots Probes and Ratiometric Analysis.Translational oncology · 2018Article
- Rinsing paired-agent model (RPAM) to quantify cell-surface receptor concentrations in topical staining applications of thick tissues.Physics in medicine and biology · 2017Article
- Generalized paired-agent kinetic model for in vivo quantification of cancer cell-surface receptors under receptor saturation conditions.Physics in medicine and biology · 2017Article
- Preclinical PET imaging of EGFR levels: pairing a targeting with a non-targeting Sel-tagged Affibody-based tracer to estimate the specific uptake.EJNMMI research · 2016Article
- Image-derived arterial input function for quantitative fluorescence imaging of receptor-drug binding in vivo.Journal of biophotonics · 2016Article
- Quantitative in vivo cell-surface receptor imaging in oncology: kinetic modeling and paired-agent principles from nuclear medicine and optical imaging.Physics in medicine and biology · 2015Review
- Quantitative in vivo immunohistochemistry of epidermal growth factor receptor using a receptor concentration imaging approach.Cancer research · 2014Article
- Microscopic lymph node tumor burden quantified by macroscopic dual-tracer molecular imaging.Nature medicine · 2014Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Dual-tracer molecular imaging is a powerful approach to quantify receptor expression in a wide range of tissues by using an untargeted tracer to account for any nonspecific uptake of a molecular-targeted tracer. This approach has previously required the pharmacokinetics of the receptor-targeted and untargeted tracers to be identical, requiring careful selection of an ideal untargeted tracer for any given targeted tracer. In this study, methodology capable of correcting for tracer differences in arterial input functions, as well as binding-independent delivery and retention, is derived and evaluated in a mouse U251 glioma xenograft model using an Affibody tracer targeted to epidermal growth factor receptor (EGFR), a cell membrane receptor overexpressed in many cancers. Simulations demonstrated that blood, and to a lesser extent vascular-permeability, pharmacokinetic differences between targeted and untargeted tracers could be quantified by deconvolving the uptakes of the two tracers in a region of interest devoid of targeted tracer binding, and therefore corrected for, by convolving the uptake of the untargeted tracer in all regions of interest by the product of the deconvolution. Using fluorescently labeled, EGFR-targeted and untargeted Affibodies (known to have different blood clearance rates), the average tumor concentration of EGFR in four mice was estimated using dual-tracer kinetic modeling to be 3.9 ± 2.4 nM compared to an expected concentration of 2.0 ± 0.4 nM. However, with deconvolution correction a more equivalent EGFR concentration of 2.0 ± 0.4 nM was measured.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.