ArticleRetrovirology2014
Asynchronous transcriptional silencing of individual retroviral genomes in embryonic cells.
Article in Retrovirology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Related Endogenous Retrovirus-K Elements Harbor Distinct Protease Active Site Motifs.Frontiers in microbiology · 2018Article
- Transposable elements in the mammalian embryo: pioneers surviving through stealth and service.Genome biology · 2016Review
- Retroviral transcriptional regulation and embryonic stem cells: war and peace.Molecular and cellular biology · 2015Review
- The KRAB zinc finger protein ZFP809 is required to initiate epigenetic silencing of endogenous retroviruses.Genes & development · 2015Article
- New bioinformatic tool for quick identification of functionally relevant endogenous retroviral inserts in human genome.Cell cycle (Georgetown, Tex.) · 2015Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundRetroviral DNAs are profoundly silenced at the transcriptional level in embryonic cell types. The transcriptional profile of pluripotent stem cells has been demonstrated to be extremely heterogeneous from cell to cell, and how the silencing of retroviral DNAs is achieved is not yet well characterized.
resultsIn the current study, we investigated the transcriptional silencing dynamics in stem cells by independently monitoring the expression of two Moloney murine leukemia virus (MMLV) retroviral vectors newly introduced into embryonic carcinoma (EC) cells. Although MMLV is efficiently silenced by epigenetic mechanisms in most such cells, a small number of the doubly-transduced EC cells transiently show double-positive proviral expression. These cells were sorted and their expression patterns were studied over time as silencing is established.
conclusionsOur data suggest that retroviral silencing occurs stochastically, in an individual locus-specific fashion, and often without synchronous silencing of both viruses in the same cells. Surprisingly, the chromatin modifications that mark the silenced proviruses are unchanged even in cells that temporarily escape silencing. This local silencing effect is a feature of stem cell epigenomic regulation that has not previously been revealed.
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Registered trials
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