ArticleBiochemistry2014
Dynamic inhomogeneity in the photodynamics of cyanobacterial phytochrome Cph1.
Article in Biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- PHY domain governs structural and photochemical fidelity in the far-red-absorbing state of phytochromes.Frontiers in molecular biosciences · 2026Article
- Photoisomerization of phytochrome's chromophore: a vibrational spectroscopic view on the primary ground state processes.RSC advances · 2025Article
- Ultrafast, remote-controlled protonation reaction enables structural changes in a phytochrome.Science advances · 2025Article
- Circular dichroism spectroscopy reveals multiple phytochrome photoproducts in equilibrium.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2025Article
- A comparative study of femtosecond pulsed and continuous wave lasers on physiological responses through activation of phytochromes in seeds.Scientific reports · 2025Article
- Cyanobacteriochromes: A Rainbow of Photoreceptors.Annual review of microbiology · 2024Review
- Two Distinct Molecular Types of Phytochrome A in Plants: Evidence of Existence and Implications for Functioning.International journal of molecular sciences · 2023Review
- Photogrammetry of Ultrafast Excited-State Intramolecular Proton Transfer Pathways in the Fungal Pigment Draconin Red.Molecules (Basel, Switzerland) · 2023Article
- Conserved histidine and tyrosine determine spectral responses through the water network in Deinococcus radiodurans phytochrome.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Enhancing the Inhomogeneous Photodynamics of Canonical Bacteriophytochrome.The journal of physical chemistry. B · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Ultrafast Photoconversion Dynamics of the Knotless PhytochromeInternational journal of molecular sciences · 2021Article
- Effect of the PHY Domain on the Photoisomerization Step of the Forward PChemistry (Weinheim an der Bergstrasse, Germany) · 2020Article
- Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein.Nature communications · 2020Article
- The Origin of Ultrafast Multiphasic Dynamics in Photoisomerization of Bacteriophytochrome.The journal of physical chemistry letters · 2020Article
- The interplay between chromophore and protein determines the extended excited state dynamics in a single-domain phytochrome.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Evolution-inspired design of multicolored photoswitches from a single cyanobacteriochrome scaffold.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Spectral and photochemical diversity of tandem cysteine cyanobacterial phytochromes.The Journal of biological chemistry · 2020Article
- Modulation of Structural Heterogeneity Controls Phytochrome Photoswitching.Biophysical journal · 2020Article
- Protochromic absorption changes in the two-cysteine photocycle of a blue/orange cyanobacteriochrome.The Journal of biological chemistry · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Phytochromes are widespread red/far-red photosensory proteins well known as critical regulators of photomorphogenesis in plants. It is often assumed that natural selection would have optimized the light sensing efficiency of phytochromes to minimize nonproductive photochemical deexcitation pathways. Surprisingly, the quantum efficiency for the forward Pr-to-Pfr photoconversion of phytochromes seldom exceeds 15%, a value very much lower than that of animal rhodopsins. Exploiting ultrafast excitation wavelength- and temperature-dependent transient absorption spectroscopy, we resolve multiple pathways within the ultrafast photodynamics of the N-terminal PAS-GAF-PHY photosensory core module of cyanobacterial phytochrome Cph1 (termed Cph1Δ) that are primarily responsible for the overall low quantum efficiency. This inhomogeneity primarily reflects a long-lived fluorescent subpopulation that exists in equilibrium with a spectrally distinct, photoactive subpopulation. The fluorescent subpopulation is favored at elevated temperatures, resulting in anomalous excited-state dynamics (slower kinetics at higher temperatures). The spectral and kinetic behavior of the fluorescent subpopulation strongly resembles that of the photochemically compromised and highly fluorescent Y176H variant of Cph1Δ. We present an integrated, heterogeneous model for Cph1Δ that is based on the observed transient and static spectroscopic signals. Understanding the molecular basis for this dynamic inhomogeneity holds potential for rational design of efficient phytochrome-based fluorescent and photoswitchable probes.
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