ArticleJournal of industrial microbiology & biotechnology2014
The novel Shewanella putrefaciens-infecting bacteriophage Spp001: genome sequence and lytic enzymes.
Article in Journal of industrial microbiology & biotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Antibacterial Activity of a Fused Endolysin ENDO-1252/KL9P Against Multiple Serovars of Salmonella enterica.Microbial biotechnology · 2025Article
- Limitation of the Lytic Effect of Bacteriophages onInternational journal of microbiology · 2025Review
- Transcriptome Reveals Regulation of Quorum Sensing ofFoods (Basel, Switzerland) · 2024Article
- A Novel Phage Indirectly Regulates Diatom Growth by Infecting a Diatom-Associated Biofilm-Forming Bacterium.Applied and environmental microbiology · 2022Article
- Bacteriophage-encoded lethal membrane disruptors: Advances in understanding and potential applications.Frontiers in microbiology · 2022Review
- Characterization of a broad-spectrum endolysin LysSP1 encoded by a Salmonella bacteriophage.Applied microbiology and biotechnology · 2021Article
- Decoding the molecular properties of mycobacteriophage D29 Holin provides insights into Holin engineering.Journal of virology · 2021Article
- Isolation and Characterization ofFrontiers in microbiology · 2020Article
- A Novel Benthic Phage InfectingViruses · 2019Article
- A major-capsid-protein-based multiplex PCR assay for rapid identification of selected virulent bacteriophage types.Archives of virology · 2019Article
- Potential for Bacteriophage Endolysins to Supplement or Replace Antibiotics in Food Production and Clinical Care.Antibiotics (Basel, Switzerland) · 2018Review
- Isolation and Characterization of a Shewanella Phage-Host System from the Gut of the Tunicate, Ciona intestinalis.Viruses · 2017Article
- Holins in bacteria, eukaryotes, and archaea: multifunctional xenologues with potential biotechnological and biomedical applications.Journal of bacteriology · 2015Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shewanella putrefaciens has been identified as a specific spoilage organism commonly found in chilled fresh fish, which contributes to the spoilage of fish products. Limiting S. putrefaciens growth can extend the shelf-life of chilled fish. Endolysins, which are lytic enzymes produced by bacteriophages, have been considered an alternative to control bacterial growth, and have been useful in various applications, including food preservation. We report here, for the first time, the complete genome sequence of a novel phage Spp001, which lyses S. putrefaciens Sp225. The Spp001 genome comprises a 54,789-bp DNA molecule with 67 open reading frames and an average total G + C content of 49.42 %. In silico analysis revealed that the Spp001 open reading frames encode various putative functional proteins, including an endolysin (ORF 62); however, no sequence for genes encoding the holin polypeptides, which work in concert with endolysins, was identified. To examine further the lytic activity of Spp001, we analyzed the lytic enzyme-containing fraction from phages released at the end of the phage lytic cycle in S. putrefaciens, using diffusion and turbidimetric assays. The results show that the partially purified extract contained endolysin, as indicated by a high hydrolytic activity towards bacterial peptidoglycan decrease in the OD590 value by 0.160 in 15 min. The results will allow further investigation of the purification of natural Spp001 endolysin, the extension of Spp001 host range, and the applications of the phage-encoded enzymes.
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