ArticleBiology of reproduction2014
Adenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm.
Article in Biology of reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Changes in the Adenylate Kinase Activity are Proportional to the ADP/ATP Ratio Upon Resorption and Regeneration of Chlamydomonas reinhardtii Flagella.Cell biochemistry and biophysics · 2025Article
- Article
- Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes.Science China. Life sciences · 2024Article
- Label-free proteomic comparison reveals ciliary and nonciliary phenotypes of IFT-A mutants.Molecular biology of the cell · 2024Article
- Adenylate kinase 9 is essential for sperm function and male fertility in mammals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Deficiency in AK9 causes asthenozoospermia and male infertility by destabilising sperm nucleotide homeostasis.EBioMedicine · 2023Article
- Regulation of Adenine Nucleotide Metabolism by Adenylate Kinase Isozymes: Physiological Roles and Diseases.International journal of molecular sciences · 2023Review
- The association of ODF4 with AK1 and AK2 in mice is essential for fertility through its contribution to flagellar shape.Scientific reports · 2023Article
- Effects of paternal methionine supplementation on sperm DNA methylation and embryo transcriptome in sheep.Environmental epigenetics · 2023Article
- The Effect of Semen Cryopreservation Process on Metabolomic Profiles of Turkey Sperm as Assessed by NMR Analysis.Biology · 2022Article
- Caput Ligation Renders Immature Mouse Sperm Motile and Capable to Undergo cAMP-Dependent Phosphorylation.International journal of molecular sciences · 2021Article
- Arrdc4-dependent extracellular vesicle biogenesis is required for sperm maturation.Journal of extracellular vesicles · 2021Article
- The genetic architecture of morphological abnormalities of the sperm tail.Human genetics · 2021Review
- CFAP45 deficiency causes situs abnormalities and asthenospermia by disrupting an axonemal adenine nucleotide homeostasis module.Nature communications · 2020Article
- Genetic causes of male infertility: snapshot on morphological abnormalities of the sperm flagellum.Basic and clinical andrology · 2019Article
- Regulatory processes that control haploid expression of salmon sperm mRNAs.BMC research notes · 2018Article
- Subcellular localization and characterization of estrogenic pathway regulators and mediators in Atlantic salmon spermatozoal cells.Histochemistry and cell biology · 2018Article
- Targeted disruption of glycogen synthase kinase 3A (GSK3A) in mice affects sperm motility resulting in male infertility.Biology of reproduction · 2015Article
- Spermatozoa from male mice with infertility due toReproductive medicine and biologyArticle
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
While most ATP, the main energy source driving sperm motility, is derived from glycolysis and oxidative phosphorylation, the metabolic demands of the cell require the efficient use of power stored in high-energy phosphate bonds. In times of high energy consumption, adenylate kinase (AK) scavenges one ATP molecule by transphosphorylation of two molecules of ADP, simultaneously yielding one molecule of AMP as a by-product. Either ATP or ADP supported motility of detergent-modeled cauda epididymal mouse sperm, indicating that flagellar AKs are functional. However, the ensuing flagellar waveforms fueled by ATP or ADP were qualitatively different. Motility driven by ATP was rapid but restricted to the distal region of the sperm tail, whereas ADP produced slower and more fluid waves that propagated down the full flagellum. Characterization of wave patterns by tracing and superimposing the images of the flagella, quantifying the differences using digital image analysis, and computer-assisted sperm analysis revealed differences in the amplitude, periodicity, and propagation of the waves between detergent-modeled sperm treated with either ATP or ADP. Surprisingly, addition of AMP to the incubation medium containing ATP recapitulated the pattern of sperm motility seen with ADP alone. In addition to AK1 and AK2, which we previously demonstrated are present in outer dense fibers and mitochondrial sheath of the mouse sperm tail, we show that another AK, AK8, is present in a third flagellar compartment, the axoneme. These results extend the known regulators of sperm motility to include AMP, which may be operating through an AMP-activated protein kinase.
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Registered trials
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