ArticleJournal of clinical microbiology2014
Inhibition controls for qualitative real-time PCR assays: are they necessary for all specimen matrices?
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Comparative Evaluation of Rapid Nucleic Acids Extraction Methods for Biosensor-Based Point-of-Care Solutions.Biosensors · 2026Article
- Advances in rapid detection technologies for zoonotic diseases: a one health-oriented review.Frontiers in cellular and infection microbiology · 2026Review
- Establishment and validation of a simple and accurate qPCR detection method for Haemophilus parasuis.Scientific reports · 2025Article
- Article
- Evaluation of molecular mouse sepsis panel: new portable and rapid tests for microorganism detection in suspected blood stream infection.Frontiers in cellular and infection microbiology · 2025Article
- Shaken, not stirred: magnetic bead DNA extraction as a rapid and effective method for the scaling up of bovine tuberculosis diagnosis.BMC veterinary research · 2024Article
- Development of advanced control material for reverse transcription-mediated bacterial nucleic acid amplification tests.Journal of clinical microbiology · 2024Article
- HCV RNA Quantification by a Domestic Commercial Assay: A Case Study among People Who Inject Drugs in Vietnam.Diagnostics (Basel, Switzerland) · 2023Article
- Description, validation, and review of a decade of experience with a laboratory-developed PCR test for detection ofJournal of clinical tuberculosis and other mycobacterial diseases · 2022Article
- The Matrix Effect in the RT-PCR Detection of SARS-CoV-2 Using Saliva without RNA Extraction.Diagnostics (Basel, Switzerland) · 2022Article
- Impact of Thermal Pretreatment of Saliva on the RT-PCR Detection of SARS-CoV-2.Advances in virology · 2022Article
- Hydrogel particles improve detection of SARS-CoV-2 RNA from multiple sample types.Scientific reports · 2020Article
- Development of a New Internally Controlled One-Step Real-Time RT-PCR for the Molecular Detection of Enterovirus A71 in Africa and Madagascar.Frontiers in microbiology · 2020Article
- Evaluation of a Novel Multiplex High-Definition PCR Assay for Detection of Tick-Borne Pathogens in Whole-Blood Specimens.Journal of clinical microbiology · 2019Article
- A sensitive gold-nanorods-based nanobiosensor for specific detection of Campylobacter jejuni and Campylobacter coli.Journal of nanobiotechnology · 2019Article
- Article
- Competition Between Strains ofFrontiers in cellular and infection microbiology · 2019Article
- Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.PloS one · 2019Article
- BaiCD gene cluster abundance is negatively correlated with Clostridium difficile infection.PloS one · 2018Article
- The abundance of the Lyme disease pathogen Borrelia afzelii declines over time in the tick vector Ixodes ricinus.Parasites & vectors · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A retrospective analysis of 386,706 specimens representing a variety of matrix types used in qualitative real-time PCR assays determined the overall inhibition rate to be 0.87% when the inhibition control was added preextraction to 5,613 specimens and 0.01% when the inhibition control was added postextraction but preamplification in 381,093 specimens. Inhibition rates of ≤ 1% were found for all specimen matrix types except urine and formalin-fixed, paraffin-embedded tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.