ArticlePloS one2014
Basic helix-loop-helix transcription factor Bmsage is involved in regulation of fibroin H-chain gene via interaction with SGF1 in Bombyx mori.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 60 citations in OpenAlex.
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- The Tra/Dsx-JHBP axis controls female-specific gene expression and oviposition in locusts.PLoS biology · 2025Article
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- Cuticle Protein LmACP19 Is Required for the Stability of Epidermal Cells in Wing Development and Morphogenesis ofInternational journal of molecular sciences · 2022Article
- Co-Expression Network and Time-Course Expression Analyses to Identify Silk Protein Regulatory Factors inInsects · 2022Article
- EnhancedInsects · 2021Article
- Genome-wide survey and characterization of transcription factors in the silk gland of the silkworm, Bombyx mori.PloS one · 2021Article
- Secrets of secretion-How studies of the Drosophila salivary gland have informed our understanding of the cellular networks underlying secretory organ form and function.Current topics in developmental biology · 2021Article
- Transgenic Ectopic Overexpression of Broad Complex (Insects · 2020Article
- Deep Insight into the Transcriptome of the Single Silk Gland ofInternational journal of molecular sciences · 2019Article
- Article
- Insect Transcription Factors: A Landscape of Their Structures and Biological Functions inInternational journal of molecular sciences · 2018Review
- New insight into the mechanism underlying the silk gland biological process by knocking out fibroin heavy chain in the silkworm.BMC genomics · 2018Article
- Genome-wide open chromatin regions and their effects on the regulation of silk protein genes in Bombyx mori.Scientific reports · 2017Article
- RNA-seq of Rice Yellow Stem BorerG3 (Bethesda, Md.) · 2017Article
- Regulation of Silk Genes by Hox and Homeodomain Proteins in the Terminal Differentiated Silk Gland of the Silkworm Bombyx mori.Journal of developmental biology · 2016Review
- In vivo fluorescence correlation spectroscopy analyses of FMBP-1, a silkworm transcription factor.FEBS open bio · 2016Article
- A juvenile hormone transcription factor Bmdimm-fibroin H chain pathway is involved in the synthesis of silk protein in silkworm, Bombyx mori.The Journal of biological chemistry · 2015Article
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10 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Silk glands are specialized in the synthesis of several secretory proteins. Expression of genes encoding the silk proteins in Bombyx mori silk glands with strict territorial and developmental specificities is regulated by many transcription factors. In this study, we have characterized B. mori sage, which is closely related to sage in the fruitfly Drosophila melanogaster. It is termed Bmsage; it encodes transcription factor Bmsage, which belongs to the Mesp subfamily, containing a basic helix-loop-helix motif. Bmsage transcripts were detected specifically in the silk glands of B. mori larvae through RT-PCR analysis. Immunoblotting analysis confirmed the Bmsage protein existed exclusively in B. mori middle and posterior silk gland cells. Bmsage has a low level of expression in the 4th instar molting stages, which increases gradually in the 5th instar feeding stages and then declines from the wandering to the pupation stages. Quantitative PCR analysis suggested the expression level of Bmsage in a high silk strain was higher compared to a lower silk strain on day 3 of the larval 5th instar. Furthermore, far western blotting and co-immunoprecipitation assays showed the Bmsage protein interacted with the fork head transcription factor silk gland factor 1 (SGF1). An electrophoretic mobility shift assay showed the complex of Bmsage and SGF1 proteins bound to the A and B elements in the promoter of fibroin H-chain gene(fib-H), respectively. Luciferase reporter gene assays confirmed the complex of Bmsage and SGF1 proteins increased the expression of fib-H. Together, these results suggest Bmsage is involved in the regulation of the expression of fib-H by being together with SGF1 in B. mori PSG cells.
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