ArticlePloS one2014
Dynamic localization of glucokinase and its regulatory protein in hypothalamic tanycytes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It carries an expression of concern. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Sugar-sensing swodkoreceptors and swodkocrine signaling.Animal models and experimental medicine · 2025Article
- Expression of Concern: Dynamic Localization of Glucokinase and Its Regulatory Protein in Hypothalamic Tanycytes.PloS one · 2025Article
- Dorzagliatin: A Breakthrough Glucokinase Activator Coming on Board to Treat Diabetes Mellitus.Cureus · 2024Review
- Glucokinase as an emerging anti-diabetes target and recent progress in the development of its agonists.Journal of enzyme inhibition and medicinal chemistry · 2022Review
- GKRP-dependent modulation of feeding behavior by tanycyte-released monocarboxylates.Theranostics · 2022Article
- Purinergic signaling in tanycytes and its contribution to nutritional sensing.Purinergic signalling · 2021Review
- Lactate activates hypothalamic POMC neurons by intercellular signaling.Scientific reports · 2021Article
- Inhibition of Hypothalamic MCT4 and MCT1-MCT4 Expressions Affects Food Intake and Alters Orexigenic and Anorexigenic Neuropeptide Expressions.Molecular neurobiology · 2020Article
- Brain Glucose-Sensing Mechanism and Energy Homeostasis.Molecular neurobiology · 2019Review
- Are Tanycytes the Missing Link Between Type 2 Diabetes and Alzheimer's Disease?Molecular neurobiology · 2019Review
- When a Little Bit More Makes the Difference: Expression Levels of GKRP Determines the Subcellular Localization of GK in Tanycytes.Frontiers in neuroscience · 2019Article
- Nutrient Sensing by Hypothalamic Tanycytes.Frontiers in endocrinology · 2019Review
- Tanycyte Gene Expression Dynamics in the Regulation of Energy Homeostasis.Frontiers in endocrinology · 2019Review
- Glucose Increase DAGLα Levels in Tanycytes and Its Inhibition Alters Orexigenic and Anorexigenic Neuropeptides Expression in Response to Glucose.Frontiers in endocrinology · 2019Article
- Cellular fate decisions in the developing female anteroventral periventricular nucleus are regulated by canonical Notch signaling.Developmental biology · 2018Article
- Glial hypothalamic inhibition of GLUT2 expression alters satiety, impacting eating behavior.Glia · 2018Article
- Connexin-43 Gap Junctions Are Responsible for the Hypothalamic Tanycyte-Coupled Network.Frontiers in cellular neuroscience · 2018Article
- Adenovirus-mediated suppression of hypothalamic glucokinase affects feeding behavior.Scientific reports · 2017Article
- Inhibition of hypothalamic MCT1 expression increases food intake and alters orexigenic and anorexigenic neuropeptide expression.Scientific reports · 2016Article
- Insights into the role of neuronal glucokinase.American journal of physiology. Endocrinology and metabolism · 2016Review
Corrections and comments
- Expression of concern
Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucokinase (GK), the hexokinase involved in glucose sensing in pancreatic β cells, is also expressed in hypothalamic tanycytes, which cover the ventricular walls of the basal hypothalamus and are implicated in an indirect control of neuronal activity by glucose. Previously, we demonstrated that GK was preferentially localized in tanycyte nuclei in euglycemic rats, which has been reported in hepatocytes and is suggestive of the presence of the GK regulatory protein, GKRP. In the present study, GK intracellular localization in hypothalamic and hepatic tissues of the same rats under several glycemic conditions was compared using confocal microscopy and Western blot analysis. In the hypothalamus, increased GK nuclear localization was observed in hyperglycemic conditions; however, it was primarily localized in the cytoplasm in hepatic tissue under the same conditions. Both GK and GKRP were next cloned from primary cultures of tanycytes. Expression of GK by Escherichia coli revealed a functional cooperative protein with a S0.5 of 10 mM. GKRP, expressed in Saccharomyces cerevisiae, inhibited GK activity in vitro with a Ki 0.2 µM. We also demonstrated increased nuclear reactivity of both GK and GKRP in response to high glucose concentrations in tanycyte cultures. These data were confirmed using Western blot analysis of nuclear extracts. Results indicate that GK undergoes short-term regulation by nuclear compartmentalization. Thus, in tanycytes, GK can act as a molecular switch to arrest cellular responses to increased glucose.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.