Evidence map›Paper›PMID 24739804›Full record

ArticlePloS one2014

Multi-method approach for characterizing the interaction between Fusarium verticillioides and Bacillus thuringiensis subsp. Kurstaki.

Liliana O Rocha, Sabina Moser Tralamazza, Gabriela M Reis, Leon Rabinovitch, Cynara B Barbosa, Benedito Corrêa

Erratum issuedAbstract read
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In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. The antagonistic effects ofCurrent medical mycology · 2016
    Article
  4. Frontiers in microbiology · 2016
    Article
  5. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors.

Liliana O RochaDepartment of Microbiology, Laboratory of Mycotoxins and Toxigenic Fungi, University of São Paulo, São Paulo, São Paulo, Brazil.
Sabina Moser TralamazzaDepartment of Microbiology, Laboratory of Mycotoxins and Toxigenic Fungi, University of São Paulo, São Paulo, São Paulo, Brazil.
Gabriela M ReisDepartment of Microbiology, Laboratory of Mycotoxins and Toxigenic Fungi, University of São Paulo, São Paulo, São Paulo, Brazil.
Leon RabinovitchDepartment of Bacteriology, Laboratory of Bacterial Physiology, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Rio de Janeiro, Brazil.
Cynara B BarbosaDepartment of Microbiology, Laboratory of Mycotoxins and Toxigenic Fungi, University of São Paulo, São Paulo, São Paulo, Brazil.
Benedito CorrêaDepartment of Microbiology, Laboratory of Mycotoxins and Toxigenic Fungi, University of São Paulo, São Paulo, São Paulo, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial antagonists used as biocontrol agents represent part of an integrated management program to reduce pesticides in the environment. Bacillus thuringiensis is considered a good alternative as a biocontrol agent for suppressing plant pathogens such as Fusarium. In this study, we used microscopy, flow cytometry, indirect immunofluorescence, and high performance liquid chromatography to determine the interaction between B. thuringiensis subsp. kurstaki LFB-FIOCRUZ (CCGB) 257 and F. verticillioides MRC 826, an important plant pathogen frequently associated with maize. B. thuringiensis showed a strong in vitro suppressive effect on F. verticillioides growth and inhibited fumonisin production. Flow cytometry analysis was found to be adequate for characterizing the fungal cell oscillations and death during these interactions. Further studies of the antagonistic effect of this isolate against other fungi and in vivo testing are necessary to determine the efficacy of B. thuringiensis subsp. kurstaki in controlling plant pathogens. This is the first report on the use of flow cytometry for quantifying living and apoptotic F. verticillioides cells and the B. thuringiensis Cry 1Ab toxin.

Indexed as

Microbial InteractionsApoptosisBacillus thuringiensisBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsFlow CytometryFusariumHemolysin ProteinsMicroscopy, Electron, TransmissionBacillus thuringiensis ToxinsBacterial ProteinsEndotoxinsHemolysin Proteinsinsecticidal crystal protein, Bacillus Thuringiensis

Identifiers

PMID24739804
PMCPMC3989188

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.