ArticleThe Journal of biological chemistry2014
Uracil-DNA glycosylase is involved in DNA demethylation and required for embryonic development in the zebrafish embryo.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 26 citations in OpenAlex.
- Zebrafish Unga Is Required for Genomic Maintenance upon Genotoxic Stress and Male Fertility.Journal of developmental biology · 2025Article
- Characterization of Heat-labile Uracil-DNA Glycosylase fromProtein and peptide letters · 2024Article
- Article
- DNA repair genes play a variety of roles in the development of fish embryos.Frontiers in cell and developmental biology · 2023Review
- Eliminating predictable DNA off-target effects of cytosine base editor by using dual guiders including sgRNA and TALE.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- DNA methylation maintenance at the p53 locus initiates biliary-mediated liver regeneration.NPJ Regenerative medicine · 2022Article
- AP endonuclease 1 (Apex1) influences brain development linking oxidative stress and DNA repair.Cell death & disease · 2019Article
- The guanine nucleotide exchange factor Net1 facilitates the specification of dorsal cell fates in zebrafish embryos by promoting maternal β-catenin activation.Cell research · 2017Article
- Abundant DNA 6mA methylation during early embryogenesis of zebrafish and pig.Nature communications · 2016Article
- Transcription Factors and DNA Repair Enzymes Compete for Damaged Promoter Sites.The Journal of biological chemistry · 2016Article
- Detection of uracil within DNA using a sensitive labeling method for in vitro and cellular applications.Nucleic acids research · 2016Article
- Uracil-DNA Glycosylase UNG Promotes Tet-mediated DNA Demethylation.The Journal of biological chemistry · 2016Article
- Si-CSP9 regulates the integument and moulting process of larvae in the red imported fire ant, Solenopsis invicta.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uracil-DNA glycosylase (Ung) is a component of the base excision repair process and has the ability to remove uracil from U:G mispairs in DNA. However, its implications in development of vertebrate embryos are poorly understood. In this study, we found that zebrafish uracil-DNA glycosylase a (Unga) is maternally expressed at high levels and accumulated in nuclei during cleavage and blastulation periods. Knockdown of unga in zebrafish embryos causes an increase of the global DNA methylation level concomitantly with a reduction of overall transcriptional activity in the nucleus, ultimately resulting in embryonic lethality during segmentation period. Conversely, unga overexpression is sufficient to reduce the global DNA methylation level, to increase H3K4me3 and H3K27me3 marks, and to activate genome transcription. Furthermore, overexpression of unga(D132A) mRNA, encoding a mutant Unga without DNA glycosylase activity, does not affect global DNA methylation level, indicating that its involvement in DNA demethylation is dependent on its glycosylase activity. These results together suggest that Unga is implicated in postfertilization genomic DNA demethylation, zygotic gene transcription, and normal embryonic development in zebrafish.
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