ArticleAnnals of botany2014
Analysis of variation for apomictic reproduction in diploid Paspalum rufum.
Article in Annals of botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Paradigm shift in apomixis research: Targeting mutants with elements of apomixis in sexual crops.Planta · 2026Review
- Genomic consequences of residual recombination in a hybrid apomictic hickory complex.Nature communications · 2026Article
- Alternative Evolutionary Pathways inGenes · 2023Article
- Variability of Reproduction Pathways in the Central-European Populations of Hawthorns with Emphasis on Triploids.Plants (Basel, Switzerland) · 2022Article
- New facts about callose events in the young ovules of some sexual and apomictic species of the Asteraceae family.Protoplasma · 2022Article
- Environmental and Genetic Factors Affecting Apospory Expressivity in DiploidPlants (Basel, Switzerland) · 2021Article
- Differential Epigenetic Marks Are Associated with Apospory Expressivity in Diploid Hybrids ofPlants (Basel, Switzerland) · 2021Article
- Review
- Article
- Chasing the Apomictic Factors in theGenes · 2020Article
- Heterochronic reproductive developmental processes between diploid and tetraploid cytotypes of Paspalum rufum.Annals of botany · 2019Article
- Reproductive Systems inFrontiers in plant science · 2019Review
- Refinement of a clearing protocol to study crassinucellate ovules of the sugar beet (Plant methods · 2019Article
- The Rise of Apomixis in Natural Plant Populations.Frontiers in plant science · 2019Review
- Partitioning Apomixis Components to Understand and Utilize Gametophytic Apomixis.Frontiers in plant science · 2019Review
- Transient Activation of Apomixis in Sexual Neotriploids May Retain Genomically Altered States and Enhance Polyploid Establishment.Frontiers in plant science · 2018Article
- Relative DNA content in diploid, polyploid, and multiploid species of Paspalum (Poaceae) with relation to reproductive mode and taxonomy.Journal of plant research · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsThe diploid cytotype of Paspalum rufum (Poaceae) reproduces sexually and is self-sterile; however, recurrent autopolyploidization through 2n + n fertilization and the ability for reproduction via apomixis have been documented in one genotype of the species. The objectives of this work were to analyse the variation in the functionality of apomixis components in diploid genotypes of P. rufum and to identify individuals with contrasting reproductive behaviours.
methodsSamples of five individuals from each of three natural populations of P. rufum (designated R2, R5 and R6) were used. Seeds were obtained after open pollination, selfing, conspecific interploidy crosses and interspecific interploidy self-pollination induction. The reproductive behaviour of each plant was determined by using the flow cytometric seed screen (FCSS) method. Embryo sacs were cleared using a series of ethanol and methyl salicylate solutions and observed microscopically. KEY
resultsIn open pollination, all genotypes formed seeds by sexual means and no evidence of apomeiotic reproduction was detected. However, in conspecific interploidy crosses and interspecific interploidy self-pollination induction, variations in the reproductive pathways were observed. While all plants from populations R2 and R6 formed seeds exclusively by sexual means, three genotypes from the R5 population developed seeds from both meiotic and aposporous embryo sacs, and one of them (R5#49) through the complete apomictic pathway (apospory + parthenogenesis + pseudogamy). Cytoembryological observations revealed the presence of both meiotic and aposporous embryo sacs in all the genotypes analysed, suggesting that parthenogenesis could be uncoupled from apospory in some genotypes.
conclusionsThe results presented demonstrate the existence of variation in the functionality of apomixis components in natural diploid genotypes of P. rufum and have identified individuals with contrasting reproductive behaviours. Genotypes identified here can be crossed to generate segregating populations in order to study apomixis determinants at the diploid level. Moreover, analysis of their expression patterns, quantification of their transcript levels and an understanding of their regulation mechanisms could help to design new strategies for recreating apomixis in a diploid genome environment.
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