ArticleBiophysical journal2014
Collagen I self-assembly: revealing the developing structures that generate turbidity.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Mechanical cues of an interpenetrating polysaccharide matrix regulate self-assembly of collagen fibers.Science advances · 2026Article
- Real-time visualization of collagen assembly uncovers metastable properties in hierarchical organization.Nature communications · 2026Article
- Nanofibrous-Composite Hydrogels for Modulating Stem Cell Behavior.Cells, tissues, organs · 2026Review
- Development and Characterization of Hollow-Shell Collagen Microcapsules for Three-Dimensional Cell Culture.Gels (Basel, Switzerland) · 2025Article
- Water and Collagen: A Mystery Yet to Unfold.Biomacromolecules · 2025Review
- Microfluidic 3D cell culture: potential application of collagen hydrogels with an optimal dose of bioactive glasses.Scientific reports · 2025Article
- Elucidating the role of water in collagen self-assembly by isotopically modulating collagen hydration.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Chondroitin sulfate, dermatan sulfate, and hyaluronic acid differentially modify the biophysical properties of collagen-based hydrogels.Acta biomaterialia · 2024Article
- 3D Bioprinting of Neurovascular Tissue Modeling with Collagen-Based Low-Viscosity Composites.Advanced healthcare materials · 2023Article
- Lose the stress: Viscoelastic materials for cell engineering.Acta biomaterialia · 2023Review
- Self-assembly of mesoscale collagen architectures and applications in 3D cell migration.Acta biomaterialia · 2023Article
- Birefringence effect studies of collagen formed by nonenzymatic glycation using dual-retarder Mueller polarimetry.Journal of biomedical optics · 2022Article
- The Applicability of Current Turbidimetric Approaches for Analyzing Fibrin Fibers and Other Filamentous Networks.Biomolecules · 2022Article
- Variation in Hydrogel Formation and Network Structure for Telo-, Atelo- and Methacrylated Collagens.Polymers · 2022Article
- Impact of Collagen Triple Helix Structure on Melanoma Cell Invadopodia Formation and Matrix Degradation upon BRAF Inhibitor Treatment.Advanced healthcare materials · 2022Article
- Decellularized peripheral nerve as an injectable delivery vehicle for neural applications.Journal of biomedical materials research. Part A · 2022Article
- Fiber finding algorithm using stepwise tracing to identify biopolymer fibers in noisy 3D images.Biophysical journal · 2021Article
- Synthesis and characterization of chemically fueled supramolecular materials driven by carbodiimide-based fuels.Nature protocols · 2021Review
- Regulatory properties of vitronectin and its glycosylation in collagen fibril formation and collagen-degrading enzyme cathepsin K activity.Scientific reports · 2021Article
- Engineering fiber anisotropy within natural collagen hydrogels.American journal of physiology. Cell physiology · 2021Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Type I collagen gels are routinely used in biophysical studies and bioengineering applications. The structural and mechanical properties of these fibrillar matrices depend on the conditions under which collagen fibrillogenesis proceeds, and developing a fuller understanding of this process will enhance control over gel properties. Turbidity measurements have long been the method of choice for monitoring developing gels, whereas imaging methods are regularly used to visualize fully developed gels. In this study, turbidity and confocal reflectance microscopy (CRM) were simultaneously employed to track collagen fibrillogenesis and reconcile the information reported by the two techniques, with confocal fluorescence microscopy (CFM) used to supplement information about early events in fibrillogenesis. Time-lapse images of 0.5 mg/ml, 1.0 mg/ml, and 2.0 mg/ml acid-solubilized collagen I gels forming at 27°C, 32°C, and 37°C were collected. It was found that in situ turbidity measured in a scanning transmittance configuration was interchangeable with traditional turbidity measurements using a spectrophotometer. CRM and CFM were employed to reveal the structures responsible for the turbidity that develops during collagen self-assembly. Information from CRM and transmittance images was collapsed into straightforward single variables; total intensity in CRM images tracked turbidity development closely for all collagen gels investigated, and the two techniques were similarly sensitive to fibril number and dimension. Complementary CRM, CFM, and in situ turbidity measurements revealed that fibril and network formation occurred before substantial turbidity was present, and the majority of increasing turbidity during collagen self-assembly was due to increasing fibril thickness.
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