ArticleBiophysical journal2014
Force generation by endocytic actin patches in budding yeast.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 73 citations in OpenAlex.
- Fast Actin Disassembly and Fimbrin Mechanosensitivity Support Rapid Turnover in a Model of Clathrin-Mediated Endocytosis.Cytoskeleton (Hoboken, N.J.) · 2025Article
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- Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Mechanistic divergences of endocytic clathrin-coated vesicle formation in mammals, yeasts and plants.Journal of cell science · 2024Review
- Lithium-induced ciliary lengthening sparks Arp2/3 complex-dependent endocytosis.Molecular biology of the cell · 2023Article
- Initial ciliary assembly inMolecular biology of the cell · 2023Article
- A model of actin-driven endocytosis explains differences of endocytic motility in budding and fission yeast.Molecular biology of the cell · 2022Article
- Endocytic proteins with prion-like domains form viscoelastic condensates that enable membrane remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Value of models for membrane budding.Current opinion in cell biology · 2021Review
- Article
- Endocytosis against high turgor pressure is made easier by partial coating and freely rotating base.Biophysical journal · 2021Article
- From Protein Building Blocks to Functional Materials.ACS nano · 2021Review
- Role of Clathrin and Dynamin in Clathrin Mediated Endocytosis/Synaptic Vesicle Recycling and Implications in Neurological Diseases.Frontiers in cellular neuroscience · 2021Review
- Article
- Single-molecule turnover dynamics of actin and membrane coat proteins in clathrin-mediated endocytosis.eLife · 2019Article
- Pulling-force generation by ensembles of polymerizing actin filaments.Physical biology · 2019Article
- Thermodynamically consistent treatment of the growth of a biopolymer in the presence of a smooth obstacle interaction potential.Physical review. E · 2019Article
- AP3M harbors actin filament binding activity that is crucial for vacuole morphology and stomatal closure inProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Actin assembly produces sufficient forces for endocytosis in yeast.Molecular biology of the cell · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Membrane deformation during endocytosis in yeast is driven by local, templated assembly of a sequence of proteins including polymerized actin and curvature-generating coat proteins such as clathrin. Actin polymerization is required for successful endocytosis, but it is not known by what mechanisms actin polymerization generates the required pulling forces. To address this issue, we develop a simulation method in which the actin network at the protein patch is modeled as an active gel. The deformation of the gel is treated using a finite-element approach. We explore the effects and interplay of three different types of force driving invagination: 1), forces perpendicular to the membrane, generated by differences between actin polymerization rates at the edge of the patch and those at the center; 2), the inherent curvature of the coat-protein layer; and 3), forces parallel to the membrane that buckle the coat protein layer, generated by an actomyosin contractile ring. We find that with optimistic estimates for the stall stress of actin gel growth and the shear modulus of the actin gel, actin polymerization can generate almost enough force to overcome the turgor pressure. In combination with the other mechanisms, actin polymerization can the force over the critical value.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.