Evidence map›Paper›PMID 24739157›Full record

ArticleBiophysical journal2014

Measuring microtubule polarity in spindles with second-harmonic generation.

Che-Hang Yu, Noah Langowitz, Hai-Yin Wu, Reza Farhadifar, Jan Brugues, Tae Yeon Yoo, Daniel Needleman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Human mitotic spindles as active liquid crystals: From collective behaviors to discrete filaments.Proceedings of the National Academy of Sciences of the United States of America · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Che-Hang YuSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts. Electronic address: chehangyu@fas.harvard.edu.
Noah LangowitzSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts.
Hai-Yin WuDepartment of Physics, Harvard University, Cambridge, Massachusetts.
Reza FarhadifarSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts.
Jan BruguesSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts; Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
Tae Yeon YooSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts.
Daniel NeedlemanSchool of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts; FAS Center for Systems Biology, Harvard University, Cambridge, Massachusetts.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The spatial organization of microtubule polarity, and the interplay between microtubule polarity and protein localization, is thought to be crucial for spindle assembly, anaphase, and cytokinesis, but these phenomena remain poorly understood, in part due to the difficulty of measuring microtubule polarity in spindles. We develop and implement a method to nonperturbatively and quantitatively measure microtubule polarity throughout spindles using a combination of second-harmonic generation and two-photon fluorescence. We validate this method using computer simulations and by comparison to structural data on spindles obtained from electron tomography and laser ablation. This method should provide a powerful tool for studying spindle organization and function, and may be applicable for investigating microtubule polarity in other systems.

Indexed as

Cell PolarityComputer SimulationAnimalsCaenorhabditis elegansCell ExtractsEmbryo, NonmammalianMicrotubulesSpindle ApparatusXenopus laevisCell Extracts

Identifiers

PMID24739157
PMCPMC4008835

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.