ArticleStem cells and development2014
Differential gene expression of the intermediate and outer interzone layers of developing articular cartilage in murine embryos.
Article in Stem cells and development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 40 citations in OpenAlex.
- Signaling and transcriptional networks governing late synovial joint development.Developmental biology · 2026Article
- Creb5 controls its own expression and directly induces the joint interzone regulatory program.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Rapid specialization and stiffening of the primitive matrix in developing articular cartilage and meniscus.Acta biomaterialia · 2023Article
- Article
- Sox, Fox, and Lmx1b binding sites differentially regulate a Gdf5-Associated regulatory region during elbow development.Frontiers in cell and developmental biology · 2023Article
- Identification of candidate enhancers controlling the transcriptome during the formation of interphalangeal joints.Scientific reports · 2022Article
- GDF5+ chondroprogenitors derived from human pluripotent stem cells preferentially form permanent chondrocytes.Development (Cambridge, England) · 2022Article
- The Genetic Epidemiology of Joint Shape and the Development of Osteoarthritis.Calcified tissue international · 2021Review
- A single cell transcriptional atlas of early synovial joint development.Development (Cambridge, England) · 2020Article
- Evolutionary Basis of High-Frequency Hearing in the Cochleae of Echolocators Revealed by Comparative Genomics.Genome biology and evolution · 2020Article
- Fetal articular cartilage regeneration versus adult fibrocartilaginous repair: secretome proteomics unravels molecular mechanisms in an ovine model.Disease models & mechanisms · 2018Article
- Cell origin, volume and arrangement are drivers of articular cartilage formation, morphogenesis and response to injury in mouse limbs.Developmental biology · 2017Article
- Articular cartilage and joint development from embryogenesis to adulthood.Seminars in cell & developmental biology · 2017Review
- Articular Cartilage: Structural and Developmental Intricacies and Questions.Current osteoporosis reports · 2015Review
- Cell-based articular cartilage repair: the link between development and regeneration.Osteoarthritis and cartilage · 2015Review
- Genesis and morphogenesis of limb synovial joints and articular cartilage.Matrix biology : journal of the International Society for Matrix Biology · 2014Review
- Fetal Cartilage Progenitor Cells in the Repair of Osteochondral Defects.JB & JS open accessArticle
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nascent embryonic joints, interzones, contain a distinct cohort of progenitor cells responsible for the formation of the majority of articular tissues. However, to date the interzone has largely been studied using in situ analysis for candidate genes in the context of the embryo rather than using an unbiased genome-wide expression analysis on isolated interzone cells, leaving significant controversy regarding the exact role of the intermediate and outer interzone layers in joint formation. Therefore, in this study, using laser capture microdissection (three biological replicates), we selectively harvested the intermediate and outer interzones of mouse embryos at gestational age 15.5 days, just prior to cavitation, when the differences between the layers should be most profound. Microarray analysis (Agilent Whole Mouse Genome Oligo Microarrays) was performed and the differential gene expression between the intermediate interzone cells and outer interzone cells was examined by performing a two-sided paired Student's t-test and pathway analysis. One hundred ninety-seven genes were differentially expressed (≥ 2-fold) between the intermediate interzone and the outer interzone with a P-value ≤ 0.01. Of these, 91 genes showed higher expression levels in the intermediate interzone and 106 were expressed higher in the outer interzone. Pathway analysis of differentially expressed genes suggests an important role for inflammatory processes in the interzone layers, especially in the intermediate interzone, and hence in joint and articular cartilage development. The high representation of genes relevant to chondrocyte hypertrophy and endochondral ossification in the outer interzone suggests that it undergoes endochondral ossification.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.