Evidence map›Paper›PMID 24738040›Full record

ArticleCancer biology & medicine2014

Interferon-alpha-2b induces autophagy in hepatocellular carcinoma cells through Beclin1 pathway.

Jun Zhao, Ming-Li Wang, Zeng Li, Dong-Mei Gao, Yu Cai, Jun Chang, Shi-Ping Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer biology & medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 17 citations in OpenAlex.

  1. Trial
  2. Review
  3. Autophagy and SARS-CoV-2-Old Players in New Games.International journal of molecular sciences · 2023
    Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Review
  9. Article
  10. Insights in Chloroquine Action: Perspectives and Implications in Malaria and COVID-19.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020
    Review
  11. Review
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Jun Zhao1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Ming-Li Wang1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Zeng Li1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Dong-Mei Gao1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Yu Cai1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Jun Chang1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Shi-Ping Wang1 Department of Parasitology, Xiangya Medical School, Central South University, Changsha 410078, China ; 2 Department of Microbiology, Anhui Medical University, Hefei 230032, China ; 3 Department of Clinical Laboratory, the Third Affiliated Hospital of Anhui Medical University, Hefei 230032, China ; 4 Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei 230032, China.
Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo determine whether Interferon-alpha-2b (IFN-α2b) can modulate the autophagic response in hepatocellular carcinoma cells.

methodsHepatocellular carcinoma cells were treated with IFN-α2b. Autophagy was assessed by acridine orange staining, GFP-LC3 dotted assay, transmission electron microscopy and immunoblotting.

resultsAcridine orange staining showed that IFN-α2b triggered the accumulation of acidic vesicular and autolysosomes in HepG2 cells. The acridine orange HepG2 cell ratios were (4.3±1.0)%, (6.9±1.4)%, and (13.1±2.3)%, respectively, after treatment with 100, 1,000, and 10,000 IU/mL IFN-α2b for 48 h. A markedly punctate pattern was observed in HepG2 cells treated with 10,000 IU/mL IFN-α2b for 48 h, but only diffuse and weakly fluorescent GFP-LC3 puncta was observed in control cells. HepG2 cells treated with 10,000 IU/mL IFN-α2b for 48 h developed autophagosome-like characteristics, including single- or double-membrane vacuoles containing intact and degraded cellular debris. The Beclin1 and LC3-II protein expression was up-regulated by IFN-α2b treatment.

conclusionAutophagy can be induced in a dose-dependent manner by treatment with IFN-α2b in HepG2 cells, and the Beclin1 signaling pathway was stimulated by IFN-α2b.

Indexed as

acridine orangeautophagyBeclin1Interferon-alpha-2b (IFN-α2b)transmission electron microscopy

Identifiers

PMID24738040
PMCPMC3969804
OpenAlexW1547571993

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.