ArticleeLife2014
Abscisic acid dynamics in roots detected with genetically encoded FRET sensors.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
93 citing papers in PubMed, 243 citations in OpenAlex.
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- ABA importers ABCG17 and ABCG18 redundantly regulate seed size in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- GIBBERELLIN PERCEPTION SENSOR 2 reveals genesis and role of cellular GA dynamics in light-regulated hypocotyl growth.The Plant cell · 2024Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- Emerging sensing, imaging, and computational technologies to scale nano-to macroscale rhizosphere dynamics - Review and research perspectives.Soil biology & biochemistry · 2024Article
- Distinct guard cell-specific remodeling of chromatin accessibility during abscisic acid- and COProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cell wall dynamics: novel tools and research questions.Journal of experimental botany · 2023Review
- Distinct guard cell specific remodeling of chromatin accessibility during abscisic acid and CObioRxiv : the preprint server for biology · 2023Article
- Spatial regulation of plant hormone action.Journal of experimental botany · 2023Review
- Highlighter: An optogenetic system for high-resolution gene expression control in plants.PLoS biology · 2023Article
- Fluorescent Biosensor Imaging of Nitrate inBio-protocol · 2023Article
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Studying root-environment interactions in structured microdevices.Journal of experimental botany · 2023Article
- Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity.Nature plants · 2023Article
- Visualising abscisic acid dynamics at low humidity in planta using ABACUS2.Nature plants · 2023Article
- Plant Hormonomics: A Key Tool for Deep Physiological Phenotyping to Improve Crop Productivity.Plant & cell physiology · 2023Review
33 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytosolic hormone levels must be tightly controlled at the level of influx, efflux, synthesis, degradation and compartmentation. To determine ABA dynamics at the single cell level, FRET sensors (ABACUS) covering a range ∼0.2-800 µM were engineered using structure-guided design and a high-throughput screening platform. When expressed in yeast, ABACUS1 detected concentrative ABA uptake mediated by the AIT1/NRT1.2 transporter. Arabidopsis roots expressing ABACUS1-2µ (Kd∼2 µM) and ABACUS1-80µ (Kd∼80 µM) respond to perfusion with ABA in a concentration-dependent manner. The properties of the observed ABA accumulation in roots appear incompatible with the activity of known ABA transporters (AIT1, ABCG40). ABACUS reveals effects of external ABA on homeostasis, that is, ABA-triggered induction of ABA degradation, modification, or compartmentation. ABACUS can be used to study ABA responses in mutants and quantitatively monitor ABA translocation and regulation, and identify missing components. The sensor screening platform promises to enable rapid fine-tuning of the ABA sensors and engineering of plant and animal hormone sensors to advance our understanding of hormone signaling. DOI: http://dx.doi.org/10.7554/eLife.01741.001.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.