ArticleeLife2014
FRET-based reporters for the direct visualization of abscisic acid concentration changes and distribution in Arabidopsis.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
102 citing papers in PubMed, 254 citations in OpenAlex.
- The architecture of salt tolerance: A multi-scale view of sodium transport in plants.Plant communications · 2026Review
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- ABA importers ABCG17 and ABCG18 redundantly regulate seed size in Arabidopsis.The Plant journal : for cell and molecular biology · 2025Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- Emerging sensing, imaging, and computational technologies to scale nano-to macroscale rhizosphere dynamics - Review and research perspectives.Soil biology & biochemistry · 2024Article
- Imaging of plant calcium-sensor kinase conformation monitors real time calcium-dependent decoding in planta.The Plant cell · 2024Article
- Distinct guard cell-specific remodeling of chromatin accessibility during abscisic acid- and COProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Distinct guard cell specific remodeling of chromatin accessibility during abscisic acid and CObioRxiv : the preprint server for biology · 2023Article
- Spatial regulation of plant hormone action.Journal of experimental botany · 2023Review
- Phosphoribosyltransferases and Their Roles in Plant Development and Abiotic Stress Response.International journal of molecular sciences · 2023Review
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Next-generation ABACUS biosensors reveal cellular ABA dynamics driving root growth at low aerial humidity.Nature plants · 2023Article
- Endosperm cellularization failure induces a dehydration-stress response leading to embryo arrest.The Plant cell · 2023Article
- Plant Hormonomics: A Key Tool for Deep Physiological Phenotyping to Improve Crop Productivity.Plant & cell physiology · 2023Review
- Optical Sensing Technologies to Elucidate the Interplay between Plant and Microbes.Micromachines · 2023Review
- Let the sunshine in: Abscisic acid modulates shade avoidance by inducing hyponasty movement in Arabidopsis.Plant physiology · 2023Article
- Plant hormone regulation of abiotic stress responses.Nature reviews. Molecular cell biology · 2022Review
- On the trail of auxin: Reporters and sensors.The Plant cell · 2022Review
- Article
- Evolutionarily conserved bacterial effectors hijack abscisic acid signaling to induce an aqueous environment in the apoplast.Cell host & microbe · 2022Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Abscisic acid (ABA) is a plant hormone that regulates plant growth and development and mediates abiotic stress responses. Direct cellular monitoring of dynamic ABA concentration changes in response to environmental cues is essential for understanding ABA action. We have developed ABAleons: ABA-specific optogenetic reporters that instantaneously convert the phytohormone-triggered interaction of ABA receptors with PP2C-type phosphatases to send a fluorescence resonance energy transfer (FRET) signal in response to ABA. We report the design, engineering and use of ABAleons with ABA affinities in the range of 100-600 nM to map ABA concentration changes in plant tissues with spatial and temporal resolution. High ABAleon expression can partially repress Arabidopsis ABA responses. ABAleons report ABA concentration differences in distinct cell types, ABA concentration increases in response to low humidity and NaCl in guard cells and to NaCl and osmotic stress in roots and ABA transport from the hypocotyl to the shoot and root. DOI: http://dx.doi.org/10.7554/eLife.01739.001.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.