ArticleThe Journal of infectious diseases2014
A role for lactate dehydrogenases in the survival of Neisseria gonorrhoeae in human polymorphonuclear leukocytes and cervical epithelial cells.
Article in The Journal of infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 34 citations in OpenAlex.
- The L-lactate dehydrogenase LldD contributes to oxidative stress resistance, survival from neutrophils, and host colonization inInfection and immunity · 2026Article
- Roles of three carbonic anhydrases inFEMS microbes · 2026Article
- Oral Neisseria gonorrhoeae Promotes KSHV Lytic Replication.Journal of medical virology · 2025Article
- Review
- The L-lactate dehydrogenases ofmBio · 2024Article
- Transcriptional responses ofmBio · 2024Article
- The L-lactate dehydrogenases ofbioRxiv : the preprint server for biology · 2024Article
- Article
- Dinner date: Neisseria gonorrhoeae central carbon metabolism and pathogenesis.Emerging topics in life sciences · 2024Review
- Transcriptome-guided metabolic network analysis reveals rearrangements of carbon flux distribution inmSystems · 2023Article
- Article
- Transcriptional regulation of theMicrobiology (Reading, England) · 2022Review
- Adherence Enables Neisseria gonorrhoeae to Overcome Zinc Limitation Imposed by Nutritional Immunity Proteins.Infection and immunity · 2022Article
- Access to highly specialized growth substrates and production of epithelial immunomodulatory metabolites determine survival of Haemophilus influenzae in human airway epithelial cells.PLoS pathogens · 2022Article
- Purification and Characterization of (2R,3R)-2,3-Butanediol Dehydrogenase of the Human Pathogen Neisseria gonorrhoeae FA1090 Produced in Escherichia coli.Molecular biotechnology · 2021Article
- Two Distinct L-Lactate Dehydrogenases Play a Role in the Survival of Neisseria gonorrhoeae in Cervical Epithelial Cells.The Journal of infectious diseases · 2020Article
- Transcriptional regulation of a gonococcal gene encoding a virulence factor (L-lactate permease).PLoS pathogens · 2019Article
- Acetate metabolic requirement of avian pathogenic Escherichia coli promotes its intracellular proliferation within macrophage.Veterinary research · 2019Article
- Deletion of D-Lactate Dehydrogenase A inFrontiers in microbiology · 2019Article
- Gonococcal Defenses against Antimicrobial Activities of Neutrophils.Trends in microbiology · 2018Review
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Lactate is an abundant metabolite, produced by host tissues and commensal organisms, and it represents an important potential carbon source for bacterial pathogens. In the case of Neisseria spp., the importance of the lactate permease in colonization of the host has been demonstrated, but there have been few studies of lactate metabolism in pathogenic Neisseria in the postgenomic era. We describe herein the characterization of genome-annotated, respiratory, and substrate-level lactate dehydrogenases (LDHs) from the obligate human pathogen Neisseria gonorrhoeae. Biochemical assays using N. gonorrhoeae 1291 wild type and isogenic mutant strains showed that cytoplasmic LdhA (NAD(+)-dependent D-lactate dehydrogenase) and the membrane-bound respiratory enzymes, LdhD (D-lactate dehydrogenase) and LldD (L-lactate dehydrogenase) are correctly annotated. Mutants lacking LdhA and LdhD showed greatly reduced survival in neutrophils compared with wild type cells, highlighting the importance of D-lactate metabolism in gonococcal survival. Furthermore, an assay of host colonization using the well-established human primary cervical epithelial cell model revealed that the two respiratory enzymes make a significant contribution to colonization of and survival within the microaerobic environment of the host. Taken together, these data suggest that host-derived lactate is critical for the growth and survival of N. gonorrhoeae in human cells.
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