ArticleEuropean spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society2015
Persistent degenerative changes in the intervertebral disc after burst fracture in an in vitro model mimicking physiological post-traumatic conditions.
Article in European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Pathobiology of Modic changes.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2016Pooled it
- [Release of moveable segments after dorsal stabilization : Impact on affected discs].Der Unfallchirurg · 2016Trial
- The healing process of vertebral body fracture in Wistar rats: creation of an animal model and demonstration of the impact of anterolateral vascularization disruption on bone healing.Journal of spine surgery (Hong Kong) · 2025Article
- Impact of traumatic intervertebral disc injury on loss of correction following pedicle screw fixation for thoracolumbar fractures.BMC musculoskeletal disorders · 2025Article
- Do we know more about the mechanobiology of the intervertebral disc in space than on Earth?JOR spine · 2025Article
- A repeated strike loading organ culture model for studying compression-associated chronic disc degeneration.Biomolecules & biomedicine · 2025Article
- Early Endoscopic Discectomy in Preventing Degenerative Spinal Changes in Patients With Lumbar Disc Herniation.Cureus · 2024Article
- Repetitive strikes loading organ culture model to investigate the biological and biomechanical responses of the intervertebral disc.JOR spine · 2024Article
- No evidence of association between either Modic change or disc degeneration and five circulating inflammatory proteins.JOR spine · 2024Article
- The role of biomechanical factors in models of intervertebral disc degeneration across multiple length scales.APL bioengineering · 2023Review
- CD90-positive stromal cells associate with inflammatory and fibrotic changes in modic changes.Osteoarthritis and cartilage open · 2022Article
- Fate of the intervertebral disc and analysis of its risk factors following high-energy traumatic thoracic and lumbar fractures: MRI results of minimum five years after injury.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2022Article
- Immuno-Modulatory Effects of Intervertebral Disc Cells.Frontiers in cell and developmental biology · 2022Review
- Programmed NP Cell Death Induced by Mitochondrial ROS in a One-Strike Loading Disc Degeneration Organ Culture Model.Oxidative medicine and cellular longevity · 2021Article
- One strike loading organ culture model to investigate the post-traumatic disc degenerative condition.Journal of orthopaedic translation · 2021Article
- Intervertebral disc damage models in organ culture: a comparison of annulus fibrosus cross-incision versus punch model under complex loading.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2018Article
- An Inflammatory Nucleus Pulposus Tissue Culture Model to Test Molecular Regenerative Therapies: Validation with Epigallocatechin 3-Gallate.International journal of molecular sciences · 2016Article
- [Injuries of the thoracolumbar spine in children].Der Orthopade · 2016Review
- Morphological changes in the human cervical intervertebral disc post trauma: response to fracture-type and degeneration grade over time.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2016Article
- Organ culture bioreactors--platforms to study human intervertebral disc degeneration and regenerative therapy.Current stem cell research & therapy · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposePost-traumatic disc degeneration (DD) is currently investigated with models not fully matching the clinical condition, in particular post-traumatic loading of the disc is not considered. Therefore, the aim was to establish an in vitro burst fracture model that more closely mimics the in vivo situation by including post-traumatic physiological loading and to investigate DD under these conditions.
methods72 rabbit spinal segments (disc/endplates + 1/3 of adjacent vertebrae) were harvested from T8/9 to L5/6 and assigned to control (n = 36) or trauma groups (n = 36). Burst fractures were induced at day 0 in the trauma group using a dropped-weight device. From day 1 to 28, all specimens were cultured at 37 °C and were dynamically loaded daily (~1 MPa nominal pressure, 1 Hz, 2,500 cycles). At day 1, 7, 14, and 28, 9 specimens from each group were taken for analysis: histology (n = 2), total disc glycosaminoglycan (GAG) content (n = 3) normalized to DNA, and qPCR of DD marker genes (n = 4) in the nucleus pulposus and the annulus fibrosus.
resultsBurst fracture with post-traumatic physiological loading resulted in a 65 % loss of GAG/DNA by day 28. Histological sections confirmed the remodeling of the matrix. Catabolic (MMP-1/-3), pro-apoptotic (TNF-α, fas ligand), and pro-inflammatory (IL-1/-6, iNOS) gene transcription was substantially up-regulated in the nucleus after the trauma and did not normalize to control within 28 days. Similar results were found for the annulus on lower levels.
conclusionAn in vitro burst fracture model with physiological post-traumatic loading was established. Under these conditions, burst spinal segments undergo strong and persistent degenerative changes.
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