ArticlePloS one2014
Identification of the major molecular types of Cryptococcus neoformans and C. gattii by Hyperbranched rolling circle amplification.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Comparative evaluation of sensititre YeastOne and VITEK2 antifungal susceptibility tests with CLSI broth microdilution method of clinicalMicrobiology spectrum · 2025Article
- Correlation BetweenJournal of fungi (Basel, Switzerland) · 2025Article
- First report of environmental isolation of Cryptococcus and Cryptococcus-like yeasts from Boyacá, Colombia.Scientific reports · 2023Article
- Development of a Real-Time PCR Assay to Identify and Distinguish betweenJournal of fungi (Basel, Switzerland) · 2022Article
- Rapid Russula senecis identification assays using loop-mediated isothermal amplification based on real-time fluorescence and visualization.Applied microbiology and biotechnology · 2022Article
- Article
- Molecular Markers Reveal Epidemiological Patterns and Evolutionary Histories of the Human PathogenicFrontiers in cellular and infection microbiology · 2021Review
- Atypical URA5 gene restriction fragment length polymorphism banding profile in Cryptococcus neoformans strains.Folia microbiologica · 2019Article
- Identification of Mycoses in Developing Countries.Journal of fungi (Basel, Switzerland) · 2019Review
- New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis.Mycopathologia · 2019Article
- Development and Evaluation of Isothermal Amplification Methods for Rapid Detection of LethalFrontiers in microbiology · 2019Article
- Molecular diagnostics in medical mycology.Nature communications · 2018Review
- Rapid Identification of Seven Waterborne Exophiala Species by RCA DNA Padlock Probes.Mycopathologia · 2018Article
- The status of cryptococcosis in Latin America.Memorias do Instituto Oswaldo Cruz · 2018Review
- The epidemiology of cryptococcosis and the characterization of Cryptococcus neoformans isolated in a Brazilian University Hospital.Revista do Instituto de Medicina Tropical de Sao Paulo · 2017Article
- Cryptococcus gattii infections.Clinical microbiology reviews · 2014Review
- Article
- Validation and clinical application of a molecular method for the identification of Cryptococcus neoformans/Cryptococcus gattii complex DNA in human clinical specimens.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious DiseasesArticle
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The agents of cryptococcosis C. neoformans and C. gattii are important agents of meningoencephalitis in immunocompromised and immunocompetent hosts, respectively. They are grouped into eight major molecular types, VNI-VNIV for C. neoformans and VGI-VGIV for C. gattii. These major molecular types differ in their host range, epidemiology, antifungal susceptibility and geographic distribution. To enable a rapid identification of the major molecular types and potential hybrids within the two species specific probes based on the PLB1 gene in combination with hyperbranched rolling circle amplification (HRCA) were developed. HRCA was applied to 76 cryptococcal strains, 10 strains each representing the 7 haploid major molecular types, 4 VNIII hybrid strains and 2 inter-species hybrid strains. All strains were correctly identified to the major molecular type and or hybrid type using HRCA alone. To increase the sensitivity a semi-nested PCR step was developed, which will enable the identification of the molecular types/hybrids directly from clinical samples, harboring a low copy number of DNA (40 copies). Thus, HRCA based on the PLB1 locus alone and in combination with a semi-nested PCR showed to be a specific and sensitive methodology, with a great potential to be used on clinical specimens for the direct diagnosis of the agents of cryptococcosis, including hybrid strains, enabling a rapid and patient tailored treatment choice of this disease.
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