ArticlePloS one2014
A modified EpiSC culture condition containing a GSK3 inhibitor can support germline-competent pluripotency in mice.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 54 citations in OpenAlex.
- Extending the residence period of allogeneic iPSC-derived invariant natural killer T cells in humanized mice by editing HLA class I expression.Frontiers in cell and developmental biology · 2026Article
- Induction of Primordial Germ Cell Like Cell from Mouse Epiblast Stem Cells in Three-Dimensional Culture.Development & reproduction · 2025Article
- Pluripotency genes of mammals: a network at work.Frontiers in bioengineering and biotechnology · 2025Review
- Relationship of PSC to embryos: Extending and refining capture of PSC lines from mammalian embryos.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Hallmarks of totipotent and pluripotent stem cell states.Cell stem cell · 2024Review
- The many dimensions of germline competence.Current opinion in cell biology · 2023Review
- Epiblast-like stem cells established by Wnt/β-catenin signaling manifest distinct features of formative pluripotency and germline competence.Cell reports · 2023Article
- Towards human organ generation using interspecies blastocyst complementation: Challenges and perspectives for therapy.Frontiers in cell and developmental biology · 2023Review
- Epigenetics as "conductor" in "orchestra" of pluripotent states.Cell and tissue research · 2022Review
- Review
- Review
- β-catenin perturbations control differentiation programs in mouse embryonic stem cells.iScience · 2022Article
- Efficient induction and sustenance of pluripotent stem cells from bovine somatic cells.Biology open · 2021Article
- Establishment of Bovine-Induced Pluripotent Stem Cells.International journal of molecular sciences · 2021Article
- Fitness selection in human pluripotent stem cells and interspecies chimeras: Implications for human development and regenerative medicine.Developmental biology · 2021Review
- Importance of WNT-dependent signaling for derivation and maintenance of primed pluripotent bovine embryonic stem cells†.Biology of reproduction · 2021Article
- The road to generating transplantable organs: from blastocyst complementation to interspecies chimeras.Development (Cambridge, England) · 2021Article
- Running the full human developmental clock in interspecies chimeras using alternative human stem cells with expanded embryonic potential.NPJ Regenerative medicine · 2021Review
- Pleiotropic roles of tankyrase/PARP proteins in the establishment and maintenance of human naïve pluripotency.Experimental cell research · 2020Review
- Domesticated cynomolgus monkey embryonic stem cells allow the generation of neonatal interspecies chimeric pigs.Protein & cell · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ESCs) can contribute to the tissues of chimeric animals, including the germline. By contrast, epiblast stem cells (EpiSCs) barely contribute to chimeras. These two types of cells are established and maintained under different culture conditions. Here, we show that a modified EpiSC culture condition containing the GSK3 inhibitor CHIR99021 can support a germline-competent pluripotent state that is intermediate between ESCs and EpiSCs. When ESCs were cultured under a modified condition containing bFGF, Activin A, and CHIR99021, they converted to intermediate pluripotent stem cells (INTPSCs). These INTPSCs were able to form teratomas in vivo and contribute to chimeras by blastocyst injection. We also induced formation of INTPSCs (iINTPSCs) from mouse embryonic fibroblasts by exogenous expression of four reprogramming factors, Oct3/4, Sox2, Klf4, and c-Myc, under the INTPSC culture condition. These iINTPSCs contributed efficiently to chimeras, including the germline, by blastocyst injection. The INTPSCs exhibited several characteristic properties of both ESCs and EpiSCs. Our results suggest that the modified EpiSC culture condition can support growth of cells that meet the most stringent criteria for pluripotency, and that germline-competent pluripotency may depend on the activation state of Wnt signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.