ArticlePloS one2014
A flow cytometry-based quantitative drug sensitivity assay for all Plasmodium falciparum gametocyte stages.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Mitochondrial ATP synthesis is essential for efficient gametogenesis in Plasmodium falciparum.Communications biology · 2024Article
- Let it glow: genetically encoded fluorescent reporters in Plasmodium.Malaria journal · 2024Review
- A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays.Communications biology · 2023Article
- Plasmodium falciparum sexual parasites regulate infected erythrocyte permeability.Communications biology · 2020Article
- Novel Method for the Separation of Male and Female Gametocytes of the Malaria ParasitemSphere · 2020Article
- Generation of NovelFrontiers in cellular and infection microbiology · 2020Article
- Screening the Pathogen Box for Molecules Active against Plasmodium Sexual Stages Using a New Nanoluciferase-Based Transgenic Line of P. berghei Identifies Transmission-Blocking Compounds.Antimicrobial agents and chemotherapy · 2018Article
- Interactions between tafenoquine and artemisinin-combination therapy partner drug in asexual and sexual stage Plasmodium falciparum.International journal for parasitology. Drugs and drug resistance · 2017Article
- The need to compare: assessing the level of agreement of three high-throughput assays against Plasmodium falciparum mature gametocytes.Scientific reports · 2017Article
- Sex-Specific Biology of the Human Malaria Parasite Revealed from the Proteomes of Mature Male and Female Gametocytes.Molecular & cellular proteomics : MCP · 2017Article
- Routine in vitro culture of P. falciparum gametocytes to evaluate novel transmission-blocking interventions.Nature protocols · 2016Article
- Large-scale production of Plasmodium falciparum gametocytes for malaria drug discovery.Nature protocols · 2016Article
- Luciferase-Based, High-Throughput Assay for Screening and Profiling Transmission-Blocking Compounds against Plasmodium falciparum Gametocytes.Antimicrobial agents and chemotherapy · 2016Article
- In Vitro Activities of Primaquine-Schizonticide Combinations on Asexual Blood Stages and Gametocytes of Plasmodium falciparum.Antimicrobial agents and chemotherapy · 2015Article
- A simple and predictive phenotypic High Content Imaging assay for Plasmodium falciparum mature gametocytes to identify malaria transmission blocking compounds.Scientific reports · 2015Article
- Sexual development in Plasmodium parasites: knowing when it's time to commit.Nature reviews. Microbiology · 2015Review
- Ensuring transmission through dynamic host environments: host-pathogen interactions in Plasmodium sexual development.Current opinion in microbiology · 2015Review
- An assay to probe Plasmodium falciparum growth, transmission stage formation and early gametocyte development.Nature protocols · 2015Article
- Effect of fluorescent dyes on in vitro-differentiated, late-stage Plasmodium falciparum gametocytes.Antimicrobial agents and chemotherapy · 2014Article
- In vitro and in vivo antimalarial activity of amphiphilic naphthothiazolium salts with amine-bearing side chains.The American journal of tropical medicine and hygiene · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundMalaria elimination/eradication campaigns emphasize interruption of parasite transmission as a priority strategy. Screening for new drugs and vaccines against gametocytes is therefore urgently needed. However, current methods for sexual stage drug assays, usually performed by counting or via fluorescent markers are either laborious or restricted to a certain stage. Here we describe the use of a transgenic parasite line for assaying drug sensitivity in all gametocyte stages.
methodsA transgenic parasite line expressing green fluorescence protein (GFP) under the control of the gametocyte-specific gene α-tubulin II promoter was generated. This parasite line expresses GFP in all gametocyte stages. Using this transgenic line, we developed a flow cytometry-based assay to determine drug sensitivity of all gametocyte stages, and tested the gametocytocidal activities of four antimalarial drugs.
findingsThis assay proved to be suitable for determining drug sensitivity of all sexual stages and can be automated. A Z' factor of 0.79 ± 0.02 indicated that this assay could be further optimized for high-throughput screening. The daily sensitivity of gametocytes to three antimalarial drugs (chloroquine, dihydroartemisinin and pyronaridine) showed a drastic decrease from stage III on, whereas it remained relatively steady for primaquine.
conclusionsA drug assay was developed to use a single transgenic parasite line for determining drug susceptibility of all gametocyte stages. This assay may be further automated into a high-throughput platform for screening compound libraries against P. falciparum gametocytes.
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