Evidence map›Paper›PMID 24735726›Full record

ArticleFEBS letters2014

Unfolding of in planta activity of anti-rep ribozyme in presence of a RNA silencing suppressor.

Sumona Karjee Mishra, Ushasri Chilakamarthi, J K Deb, Sunil Kumar Mukherjee

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 12 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Sumona Karjee MishraPlant Molecular Biology Division, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India. Electronic address: sumonakarjee@gmail.com.
Ushasri ChilakamarthiDepartment of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110 016, India.
J K DebDepartment of Biochemical Engineering and Biotechnology, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi 110 016, India.
Sunil Kumar MukherjeePlant Molecular Biology Division, International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110 067, India; Department of Genetics, University of Delhi, South Campus, Benito Juarez Marg, New Delhi 110021, India.
Indian Institute of Technology Delhi · INInternational Centre for Genetic Engineering and Biotechnology · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antisense RNA ribozymes have intrinsic endonucleolytic activity to effect cleavage of the target RNA. However, this activity in vivo is often controlled by the dominance of antisense or other double-stranded RNA mechanism. In this work, we demonstrate the in planta activity of a hammerhead ribozyme designed to target rep-mRNA of a phytopathogen Mungbean Yellow Mosaic India virus (MYMIV) as an antiviral agent. We also found RNA-silencing is induced on introduction of catalytically active as well as inactive ribozymes. Using RNA-silencing suppressors (RSS), we demonstrate that the endonucleolytic activity of ribozymes is a true phenomenon, even while a mutated version may demonstrate a similar down-regulation of the target RNA. This helps to ease the confusion over the action mechanism of ribozymes in vivo.

Indexed as

RNA InterferenceBegomovirusBlotting, NorthernEndonucleasesFabaceaeNicotianaPlant DiseasesPlant LeavesReverse Transcriptase Polymerase Chain ReactionRNA, CatalyticRNA, MessengerRNA, PlantRNA, ViralViral ProteinsEndonucleasesRNA, CatalyticRNA, MessengerRNA, PlantRNA, ViralViral ProteinsHammerhead ribozymeIn plantaMungbean Yellow Mosaic India virusRNA-silencingRNA-silencing suppressor

Identifiers

PMID24735726
PMCPMC7164126
OpenAlexW2063339598

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.