ArticleJournal of neuroscience methods2014
A novel technique for morphometric quantification of subarachnoid hemorrhage-induced microglia activation.
Article in Journal of neuroscience methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- The Complementary Role of Morphology in Understanding Microglial Functional Heterogeneity.International journal of molecular sciences · 2025Review
- Establishment of an AI-supported scoring system for neuroglial cells.Frontiers in cellular neuroscience · 2025Article
- Intravital 2-photon imaging reveals distinct morphology and infiltrative properties of glioblastoma-associated macrophages.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Central nervous system lymphatic unit, immunity, and epilepsy: Is there a link?Epilepsia open · 2019Review
- Article
- Multiple Morphometric Assessment of Microglial Cells in Deafferented Spinal Trigeminal Nucleus.Frontiers in neuroanatomy · 2019Article
- Automated Morphological Analysis of Microglia After Stroke.Frontiers in cellular neuroscience · 2018Article
- Distinction of Neurons, Glia and Endothelial Cells in the Cerebral Cortex: An Algorithm Based on Cytological Features.Frontiers in neuroanatomy · 2016Article
- Activation of mGluR5 Attenuates Microglial Activation and Neuronal Apoptosis in Early Brain Injury After Experimental Subarachnoid Hemorrhage in Rats.Neurochemical research · 2015Article
- Article
- Immune cells subpopulations in cerebrospinal fluid and peripheral blood of patients with Aneurysmal Subarachnoid Hemorrhage.SpringerPlus · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundSubarachnoid hemorrhage (SAH) is a neurologic catastrophe and poor outcome is typically attributed to vasospasm; however, there is also evidence that SAH causes a pro-inflammatory state and these two phenomena may be interrelated. SAH causes activation of microglia, but the time course and degree of microglial activation after SAH and its link to poor patient outcome and vasospasm remains unknown. NEW
methodTransgenic mice expressing eGFP under the control of the CX3CR1 locus, in which microglia are endogenously fluorescent, were randomly assigned to control or SAH groups. Immunohistochemistry for CD-68 and CD-31 was performed at different time points after SAH. Using confocal microscopy and MatLab software, we have developed a novel technique to detect and quantify the stages of microglial activation and return to quiescence using an automated computerized morphometric analysis.
resultsWe detected a statistically significant decrease in microglial process complexity 2 and 7 days following SAH. In addition, we detected a statistically significant increase in microglial domain volume 1 day following SAH; however, microglial domain volume returned to baseline by 2 days. COMPARISON WITH EXISTING
methodMost techniques for microglia assessment are qualitative, not quantitative, and are therefore inadequate to address the effects of anti-inflammatory drug treatment or other therapies after SAH.
conclusionsUsing novel image analysis techniques we were able to reproducibly quantify activation of microglia following SAH, which will improve our ability to study the biology of microglial activation, and may ultimately improve management of disease progression and response to therapies directed at microglial activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.