ArticleTissue engineering. Part C, Methods2014
Influence of pH on extracellular matrix preservation during lung decellularization.
Article in Tissue engineering. Part C, Methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Proteomic evaluation and comparison of native and decellularized human saphenous vein extracellular matrices.Frontiers in bioengineering and biotechnology · 2026Article
- Enhancing peripheral nerve regeneration through NaOH-based decellularization of human nerve tissue.Bioengineering & translational medicine · 2025Article
- Acellular Extracellular Matrix Scaffolds in Regenerative Medicine: Advances in Decellularization and Clinical Applications.Journal of functional biomaterials · 2025Review
- Advances in abiotic tissue-based biomaterials: A focus on decellularization and devitalization techniques.Materials today. Bio · 2025Article
- Decellularization techniques: unveiling the blueprint for tracheal tissue engineering.Frontiers in bioengineering and biotechnology · 2025Review
- A pilot study for decellularizing porcine cornea for future use in corneal regeneration.PloS one · 2025Article
- Application of decellularization methods for scaffold production: advantages, disadvantages, biosafety and modifications.Frontiers in bioengineering and biotechnology · 2025Review
- Development of a 3D tumor model based on decellularized matrix using high-throughput approaches.Frontiers in bioengineering and biotechnology · 2025Review
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities.Bioactive materials · 2024Review
- Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting.ACS omega · 2024Review
- Adipose-Derived Mesenchymal Stem Cells Attenuate Immune Reactions Against Pig Decellularized Bronchi Engrafted into Rat Tracheal Defects.Organogenesis · 2023Article
- Current status and future prospects of decellularized kidney tissue.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2023Review
- Development of Brain-Derived Bioscaffolds for Neural Progenitor Cell Culture.ACS pharmacology & translational science · 2023Article
- Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine.Biomaterials research · 2023Review
- Development potential of extracellular matrix hydrogels as hemostatic materials.Frontiers in bioengineering and biotechnology · 2023Review
- Clinical Translation of Engineered Pulmonary Vascular Models.Advances in experimental medicine and biology · 2023Article
- Angiogenesis and Re-endothelialization in decellularized scaffolds: Recent advances and current challenges in tissue engineering.Frontiers in bioengineering and biotechnology · 2023Review
- Decellularization in Tissue Engineering and Regenerative Medicine: Evaluation, Modification, and Application Methods.Frontiers in bioengineering and biotechnology · 2022Review
- Development of 2-D and 3-D culture platforms derived from decellularized nucleus pulposus.Frontiers in bioengineering and biotechnology · 2022Article
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Authors and funding
6 authors.
Funding
Abstract
The creation of decellularized organs for use in regenerative medicine requires the preservation of the organ extracellular matrix (ECM) as a means to provide critical cues for differentiation and migration of cells that are seeded onto the organ scaffold. The purpose of this study was to assess the influence of varying pH levels on the preservation of key ECM components during the decellularization of rat lungs. Herein, we show that the pH of the 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)-based decellularization solution influences ECM retention, cell removal, and also the potential for host response upon implantation of acellular lung tissue. The preservation of ECM components, including elastin, fibronectin, and laminin, were better retained in the lower pH conditions that were tested (pH ranges tested: 8, 10, 12); glycosaminoglycans were preserved to a higher extent in the lower pH groups as well. The DNA content following decellularization of the rat lung was inversely correlated with the pH of the decellularization solution. Despite detectible levels of cyotoskeletal proteins and significant residual DNA, tissues decellularized at pH 8 demonstrated the greatest tissue architecture maintenance and the least induction of host response of all acellular conditions. These results highlight the effect of pH on the results obtained by organ decellularization and suggest that altering the pH of the solutions used for decellularization may influence the ability of cells to properly differentiate and home to appropriate locations within the scaffold, based on the preservation of key ECM components and implantation results.
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