ArticleAllergy2014
Epicutaneously applied Der p 2 induces a strong TH 2-biased antibody response in C57BL/6 mice, independent of functional TLR4.
Article in Allergy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 37 citations in OpenAlex.
- Co-Activation of Human Whole Blood Cells with Lipopolysaccharides and an Allergen.Life (Basel, Switzerland) · 2023Article
- When the allergy alarm bells toll: The role of Toll-like receptors in allergic diseases and treatment.Frontiers in molecular biosciences · 2023Review
- Non-IgE-reactive allergen peptides deteriorate the skin barrier in house dust mite-sensitized atopic dermatitis patients.Frontiers in cell and developmental biology · 2023Article
- Atopic dermatitis trajectories to age 8 years in the GUSTO cohort.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2021Article
- Der f 38 Is a Novel TLR4-Binding Allergen Related to Allergy Pathogenesis fromInternational journal of molecular sciences · 2021Article
- Characterization of Innate Immune Responses to House Dust Mite Allergens: Pitfalls and Limitations.Frontiers in allergy · 2021Review
- Pathogenic Mechanism of Der p 38 as a Novel Allergen Homologous to RipA and RipB Proteins in Atopic Dermatitis.Frontiers in immunology · 2021Article
- Systematic Characterization of the Group 2 House Dust Mite Allergen inFrontiers in cellular and infection microbiology · 2021Article
- Insights Into Mucosal Innate Immune Responses in House Dust Mite-Mediated Allergic Asthma.Frontiers in immunology · 2020Review
- Mechanisms by Which Atopic Dermatitis Predisposes to Food Allergy and the Atopic March.Allergy, asthma & immunology research · 2019Review
- Allergy immunotherapy restores airway epithelial barrier dysfunction through suppressing IL-25 -induced endoplasmic reticulum stress in asthma.Scientific reports · 2018Article
- Clinical efficacy of sublingual immunotherapy is associated with restoration of steady-state serum lipocalin 2 after SLIT: a pilot study.The World Allergy Organization journal · 2018Article
- Etiology of epithelial barrier dysfunction in patients with type 2 inflammatory diseases.The Journal of allergy and clinical immunology · 2017Review
- International consensus (ICON) on: clinical consequences of mite hypersensitivity, a global problem.The World Allergy Organization journal · 2017Article
- House Dust Mite Allergen Regulates Constitutive Apoptosis of Normal and Asthmatic Neutrophils via Toll-Like Receptor 4.PloS one · 2015Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe major house dust mite allergen Der p 2 is a structural and functional homologue of MD-2 within the TLR4-CD14-MD-2 complex. An asthma mouse model in TLR4-deficient mice recently suggested that the allergic immune response against Der p 2 is solely dependent on TLR4 signaling. We investigated whether similar mechanisms are important for Der p 2 sensitization via the skin.
methodsIn an epicutaneous sensitization model, the response to recombinant Der p 2 in combination with or without lipopolysaccharide (LPS) was compared between C57BL/6 WT and TLR4-deficient mice. We further analyzed possible adjuvant function of exogenous cysteine proteases.
resultsSensitization with rDer p 2 induced similar levels of allergen-specific IgG1 and IgE antibodies in both mouse strains. LPS increased the systemic (antibody levels, cytokine release by restimulated splenocytes) and local (infiltration of immune cells into the skin) Th2 immune responses, which against our expectations were stronger in the absence of functional TLR4 expression. Barrier disruption by papain, a protease with structural homology to Der p 1, did not enhance the sensitization capacity of rDer p 2. However, the presence of LPS increased the stability of rDer p 2 against the protease.
conclusionOur data suggest that rDer p 2 alone can cause a strong TH 2-biased response via the skin being enhanced in the presence of LPS. This response is not reliant on functional TLR4, but vice versa TLR4 expression rather protects against epicutaneous sensitization to house dust mite allergen Der p 2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.