ArticlePloS one2014
Reducing the dietary omega-6:omega-3 utilizing α-linolenic acid; not a sufficient therapy for attenuating high-fat-diet-induced obesity development nor related detrimental metabolic and adipose tissue inflammatory outcomes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Serum Fetuin-A Affected by Nutritional Status as a Novel Biomarker for Coronary Artery Disease.Acta Cardiologica Sinica · 2025Article
- Harnessing black soldier fly (Open veterinary journal · 2024Article
- Soybean oil-based HFD induces gut dysbiosis that leads to steatosis, hepatic inflammation and insulin resistance in mice.Frontiers in microbiology · 2024Article
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- Stearidonic-Enriched Soybean Oil Modulates Obesity, Glucose Metabolism, and Fatty Acid Profiles Independently of Akkermansia muciniphila.Molecular nutrition & food research · 2020Article
- Role of microRNA in CB1 antagonist-mediated regulation of adipose tissue macrophage polarization and chemotaxis during diet-induced obesity.The Journal of biological chemistry · 2019Article
- Repeated clodronate-liposome treatment results in neutrophilia and is not effective in limiting obesity-linked metabolic impairments.American journal of physiology. Endocrinology and metabolism · 2019Article
- Effects of dietary fatty acid content on humeral cartilage and bone structure in a mouse model of diet-induced obesity.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2019Article
- High Dietary ω-6:ω-3 PUFA Ratio Is Positively Associated with Excessive Adiposity and Waist Circumference.Obesity facts · 2018Article
- Blockade of CB1 cannabinoid receptor alters gut microbiota and attenuates inflammation and diet-induced obesity.Scientific reports · 2017Article
- Article
- miR155 deficiency aggravates high-fat diet-induced adipose tissue fibrosis in male mice.Physiological reports · 2017Article
- Dietary supplementation of α-linolenic acid induced conversion of n-3 LCPUFAs and reduced prostate cancer growth in a mouse model.Lipids in health and disease · 2017Article
- EPA prevents fat mass expansion and metabolic disturbances in mice fed with a Western diet.Journal of lipid research · 2016Article
- Eicosapentaenoic acid attenuates dexamethasome-induced apoptosis by inducing adaptive autophagy via GPR120 in murine bone marrow-derived mesenchymal stem cells.Cell death & disease · 2016Article
- Role of MCP-1 on inflammatory processes and metabolic dysfunction following high-fat feedings in the FVB/N strain.International journal of obesity (2005) · 2016Article
- A Low Dose of Dietary Quercetin Fails to Protect against the Development of an Obese Phenotype in Mice.PloS one · 2016Article
- Dietary Fructose Activates Insulin Signaling and Inflammation in Adipose Tissue: Modulatory Role of Resveratrol.BioMed research international · 2016Article
- A High Linoleic Acid Diet does not Induce Inflammation in Mouse Liver or Adipose Tissue.Lipids · 2015Article
- Fish oil and flax seed oil supplemented diets increase FFAR4 expression in the rat colon.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
aimsTo examine the effect of manipulating the omega-6:omega-3 (1∶1, 5∶1, 10∶1, and 20∶1) utilizing only α-linolenic and linoleic acid within a clinically-relevant high-fat diet (HFD) composed of up to seven sources of fat and designed to be similar to the standard American diet (MUFA∶PUFA of 2∶1, 12% and 40% of calories from saturated and total fat, respectively) on body composition, macrophage polarization, inflammation, and metabolic dysfunction in mice.
methodsDiets were administered for 20 weeks. Body composition and metabolism (HOMA index and lipid profile) were examined monthly. GC-MS was utilized to determine the eicosapentaenoic acid (EPA):arachidonic acid (AA) and the docosahexaenoic acid (DHA):AA in AT phospholipids. Adipose tissue (AT) mRNA expression of chemokines (MCP-1, Fetuin-A, CXCL14), marker genes for M1 and M2 macrophages (CD11c and CD206, respectively) and inflammatory markers (TNF-α, IL-6, IL-1β, TLR-2, TLR-4, IL-10, GPR120) were measured along with activation of NFκB, JNK, and STAT-3. Macrophage infiltration into AT was examined using F4/80 immunohistochemistry.
resultsAny therapeutic benefit produced by reducing the omega-6:omega-3 was evident only when comparing the 1∶1 to 20∶1 HFD; the 1∶1 HFD resulted in a lower TC:HDL-C and decreased AT CXCL14 gene expression and AT macrophage infiltration, which was linked to a higher EPA:AA and DHA:AA in AT phospholipids. However, despite these effects, and independent of the omega-6:omega-3, all HFDs, in general, led to similar levels of adiposity, insulin resistance, and AT inflammation.
conclusionReducing the omega-6:omega-3 using α-linolenic acid is not an effective therapy for attenuating obesity and type II diabetes mellitus development.
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