ArticleYeast (Chichester, England)2014
Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast.
Article in Yeast (Chichester, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- ABA-AA: A simple, reversible, and non-toxic anchor-away system for effective nuclear protein depletion.Cell reports methods · 2026Article
- Article
- Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Production of CRISPR-Cas9 Transgenic Cell Lines for Knocksideways Studies.Current protocols · 2023Article
- The conserved RNA-binding protein Seb1 promotes cotranscriptional ribosomal RNA processing by controlling RNA polymerase I progression.Nature communications · 2023Article
- Review
- Cdc48 influence on separase levels is independent of mitosis and suggests translational sensitivity of separase.Cell reports · 2022Article
- Perturbation of kinetochore function using GFP-binding protein in fission yeast.G3 (Bethesda, Md.) · 2021Article
- Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins inG3 (Bethesda, Md.) · 2020Article
- Common mechanism of transcription termination at coding and noncoding RNA genes in fission yeast.Nature communications · 2018Article
- Regulated reconstitution of spindle checkpoint arrest and silencing through chemically induced dimerisationJournal of cell science · 2018Article
- Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.Journal of cell science · 2018Article
- Go in! Go out! Inducible control of nuclear localization.Current opinion in chemical biology · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Genetic analysis of protein function requires a rapid means of inactivating the gene under study. Typically, this exploits temperature-sensitive mutations or promoter shut-off techniques. We report the adaptation to Schizosaccharomyces pombe of the anchor-away technique, originally designed in budding yeast by Laemmli lab. This method relies on a rapamycin-mediated interaction between the FRB- and FKBP12-binding domains to relocalize nuclear proteins of interest to the cytoplasm. We demonstrate a rapid nuclear depletion of abundant proteins as proof of principle.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.