ArticlePloS one2014
Methods for the preparation of large quantities of complex single-stranded oligonucleotide libraries.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
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- Development of an isothermal point of care method for detection of human papilloma virus (HPV).Aspects of molecular medicine · 2026Article
- Origin of small chromosome A08 and genome evolution of Arachis species.Nature communications · 2026Article
- Off-target detection of CRISPR-Cas9 nucleaseSynthetic biology (Oxford, England) · 2026Article
- Cytogenetic screening of a Canadian swine breeding nucleus using a newly developed karyotyping method named oligo-banding.Genetics, selection, evolution : GSE · 2023Article
- Strategies for generation of mice via CRISPR/HDR-mediated knock-in.Molecular biology reports · 2023Review
- Bulked Oligo-FISH for Chromosome Painting and Chromosome Barcoding.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Suitability evaluation of toehold switch and EXPAR for cell-free MicroRNA biosensor development.Biotechnology notes (Amsterdam, Netherlands) · 2023Article
- Biomolecule-Based Optical Metamaterials: Design and Applications.Biosensors · 2022Review
- Homology-based repair induced by CRISPR-Cas nucleases in mammalian embryo genome editing.Protein & cell · 2022Review
- Converting Double-Stranded DNA-Encoded Libraries (DELs) to Single-Stranded Libraries for More Versatile Selections.ACS omega · 2022Article
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- FISH Going Meso-Scale: A Microscopic Search for Chromatin Domains.Frontiers in cell and developmental biology · 2021Review
- The Puzzling Fate of a Lupin Chromosome Revealed by Reciprocal Oligo-FISH and BAC-FISH Mapping.Genes · 2020Article
- Highly Multiplexed Single-Cell In Situ RNA and DNA Analysis by Consecutive Hybridization.Molecules (Basel, Switzerland) · 2020Article
- Advances in Chromatin Imaging at Kilobase-Scale Resolution.Trends in genetics : TIG · 2020Review
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- iFISH is a publically available resource enabling versatile DNA FISH to study genome architecture.Nature communications · 2019Article
- Whole-chromosome paints in maize reveal rearrangements, nuclear domains, and chromosomal relationships.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
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Authors and funding
3 authors.
Funding
Abstract
Custom-defined oligonucleotide collections have a broad range of applications in fields of synthetic biology, targeted sequencing, and cytogenetics. Also, they are used to encode information for technologies like RNA interference, protein engineering and DNA-encoded libraries. High-throughput parallel DNA synthesis technologies developed for the manufacture of DNA microarrays can produce libraries of large numbers of different oligonucleotides, but in very limited amounts. Here, we compare three approaches to prepare large quantities of single-stranded oligonucleotide libraries derived from microarray synthesized collections. The first approach, alkaline melting of double-stranded PCR amplified libraries with a biotinylated strand captured on streptavidin coated magnetic beads results in little or no non-biotinylated ssDNA. The second method wherein the phosphorylated strand of PCR amplified libraries is nucleolyticaly hydrolyzed is recommended when small amounts of libraries are needed. The third method combining in vitro transcription of PCR amplified libraries to reverse transcription of the RNA product into single-stranded cDNA is our recommended method to produce large amounts of oligonucleotide libraries. Finally, we propose a method to remove any primer binding sequences introduced during library amplification.
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