ArticleThe Journal of biological chemistry2014
Telomerase reverse transcriptase has an extratelomeric function in somatic cell reprogramming.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 2 of them syntheses that pooled it.
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Who cites it
12 citing papers in PubMed, 2 syntheses or guidelines pooled it, 17 citations in OpenAlex.
- Loci for human leukocyte telomere length in the Singaporean Chinese population and trans-ethnic genetic studies.Nature communications · 2019Pooled it
- Telomere Biology and Thoracic Aortic Aneurysm.International journal of molecular sciences · 2017Pooled it
- An Unbiased Approach to Identifying Cellular Reprogramming-Inducible Enhancers.International journal of molecular sciences · 2024Article
- Maternal stress-induced changes in adolescent and adult offspring: Neurobehavioural improvement and telomere maintenance.Heliyon · 2023Article
- The dual distinct role of telomerase in repression of senescence and myofibroblast differentiation.Aging · 2021Article
- RNA-dependent RNA polymerase, RdRP, a promising therapeutic target for cancer and potentially COVID-19.Cancer science · 2020Review
- Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications.Stem cell reviews and reports · 2020Review
- The role of telomeres and telomerase reverse transcriptase isoforms in pluripotency induction and maintenance.RNA biology · 2016Review
- Blockade of senescence-associated microRNA-195 in aged skeletal muscle cells facilitates reprogramming to produce induced pluripotent stem cells.Aging cell · 2016Article
- Telomerase reverse transcriptase moonlights: Therapeutic targets beyond telomerase.Cancer science · 2015Review
- Saccharomyces cerevisiae as a model for the study of extranuclear functions of mammalian telomerase.Current genetics · 2015Article
- Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactivation of the endogenous telomerase reverse transcriptase (TERT) catalytic subunit and telomere elongation occur during the reprogramming of somatic cells to induced pluripotent stem (iPS) cells. However, the role of TERT in the reprogramming process is unclear. To clarify its function, the reprogramming process was examined in TERT-KO somatic cells. To exclude the effect of telomere elongation, tail-tip fibroblasts (TTFs) from first generation TERT-KO mice were used. Although iPS cells were successfully generated from TERT-KO TTFs, the efficiency of reprogramming these cells was markedly lower than that of WT TTFs. The gene expression profiles of iPS cells induced from TERT-KO TTFs were similar to those of WT iPS cells and ES cells, and TERT-KO iPS cells formed teratomas that differentiated into all three germ layers. These data indicate that TERT plays an extratelomeric role in the reprogramming process, but its function is dispensable. However, TERT-KO iPS cells showed transient defects in growth and teratoma formation during continuous growth. In addition, TERT-KO iPS cells developed chromosome fusions that accumulated with increasing passage numbers, consistent with the fact that TERT is essential for the maintenance of genome structure and stability in iPS cells. In a rescue experiment, an enzymatically inactive mutant of TERT (D702A) had a positive effect on somatic cell reprogramming of TERT-KO TTFs, which confirmed the extratelomeric role of TERT in this process.
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Registered trials
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