ArticlePloS one2014
Comparison between RFLP and MIRU-VNTR genotyping of Mycobacterium tuberculosis strains isolated in Stockholm 2009 to 2011.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
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Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Laboratory Cross-Contamination of Mycobacterium tuberculosis: A Systematic Review and Meta-analysis.Lung · 2019Pooled it
- Pooled it
- Article
- Identification and Characterization of Mycobacterial Species Using Whole-Genome Sequences.Methods in molecular biology (Clifton, N.J.) · 2021Article
- False-Positive Mycobacterium tuberculosis Detection: Ways to Prevent Cross-Contamination.Tuberculosis and respiratory diseases · 2020Article
- Comparison of routine field epidemiology and whole genome sequencing to identify tuberculosis transmission in a remote setting.Epidemiology and infection · 2020Article
- Role of Immigration in Tuberculosis Transmission to Iran: A Systematic Review.International journal of preventive medicine · 2020Review
- Exploring the usefulness of molecular epidemiology of tuberculosis in Africa: a systematic review.International journal of molecular epidemiology and genetics · 2020Review
- Usefulness of 3'- 5' IS6110-RFLP genotyping and spoligotyping of Mycobacterium tuberculosis isolated in a tertiary hospital: a retrospective study detecting unsuspected epidemiological events.Revista do Instituto de Medicina Tropical de Sao Paulo · 2019Article
- Assessment of the use and need for an integrated molecular surveillance of tuberculosis: an online survey in Germany.BMC public health · 2019Article
- Molecular and genomic typing for tuberculosis surveillance: A survey study in 26 European countries.PloS one · 2019Article
- Universal genotyping reveals province-level differences in the molecular epidemiology of tuberculosis.PloS one · 2019Article
- Role of Molecular Epidemiology on Tuberculosis Control in the Middle East Countries: a Systematic Review and Meta-Analysis.Tanaffos · 2018Article
- Role of MIRU-VNTR and spoligotyping in assessing the genetic diversity of Mycobacterium tuberculosis in Henan Province, China.BMC infectious diseases · 2018Article
- The Epidemiological Significance and Temporal Stability of Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats-Based Method Applied toInternational journal of environmental research and public health · 2018Article
- Automated IS6110-based fingerprinting of Mycobacterium tuberculosis: Reaching unprecedented discriminatory power and versatility.PloS one · 2018Article
- PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping.Scientific reports · 2017Article
- Exploring genotype concordance in epidemiologically linked cases of tuberculosis in New York City.Epidemiology and infection · 2017Article
- Integration of molecular typing results into tuberculosis surveillance in Germany-A pilot study.PloS one · 2017Article
- Human tuberculosis caused by Mycobacterium bovis: a retrospective comparison with Mycobacterium tuberculosis in a Mexican tertiary care centre, 2000-2015.BMC infectious diseases · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Our aim was to analyze the difference between methods for genotyping of Mycobacterium tuberculosis complex isolates. We collected genotyping results from Restriction Fragment Length Polymorphism (RFLP) and Mycobacterial Interspersed Repetitive Units-Variable Numbers of Tandem Repeat (MIRU-VNTR) in a geographically limited area (Stockholm) during a period of three years. The number and proportion of isolates belonging to clusters was reduced by 45 and 35% respectively when combining the two methods compared with using RFLP or MIRU-VNTR only. The mean size of the clusters was smaller when combining methods and smaller with RFLP compared to MIRU-VNTR. In clusters with confirmed epidemiological links RFLP coincided slightly better than MIRU-VNTR but where there was a difference, the variation in MIRU-VNTR pattern was only in a single locus. In isolates with few IS6110 bands in RFLP, MIRU-VNTR differentiated the isolates more, dividing the RFLP clusters. Since MIRU-VNTR is faster and less labour-intensive it is the method of choice for routine genotyping. In most cases it will be sufficient for epidemiological purposes but true clustering might still be considered if there are epidemiological links and the MIRU-VNTR results differ in only one of its 24 loci.
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