ArticleInfection and immunity2014
Role of intracellular carbon metabolism pathways in Shigella flexneri virulence.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 34 citations in OpenAlex.
- Glucose-dependent acidification strategy by nectar-dwelling bacteria mediates pollen tube burst.Nature communications · 2026Article
- Adaptive metabolic strategies of intracellular bacterial pathogens.Frontiers in microbiology · 2026Review
- Whole genome analysis ofFrontiers in veterinary science · 2025Article
- (p)ppGpp is required for virulence ofInfection and immunity · 2024Article
- Metabolic Analysis of Intracellular Pathogenic Bacteria Using NMR.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Application of Proteomics to the Study of the Therapeutics and Pathogenicity ofDiagnostics (Basel, Switzerland) · 2022Review
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- Glucose Starvation, Magnesium Ion Starvation, and Bile Stress Assays.Bio-protocol · 2021Article
- Pectobacterium atrosepticum KDPG aldolase, Eda, participates in the Entner-Doudoroff pathway and independently inhibits expression of virulence determinants.Molecular plant pathology · 2021Article
- Laboratory Cultivation and Storage of Shigella.Current protocols in microbiology · 2019Article
- Gut Microbiota and Colonization Resistance against Bacterial Enteric Infection.Microbiology and molecular biology reviews : MMBR · 2019Review
- Nutrition and Bipartite Metabolism of Intracellular Pathogens.Trends in microbiology · 2019Review
- Article
- Immunometabolism at the interface between macrophages and pathogens.Nature reviews. Immunology · 2019Review
- Human Intestinal Enteroids as a Model System ofInfection and immunity · 2019Article
- How Viral and Intracellular Bacterial Pathogens Reprogram the Metabolism of Host Cells to Allow Their Intracellular Replication.Frontiers in cellular and infection microbiology · 2019Review
- A requirement for septins and the autophagy receptor p62 in the proliferation of intracellular Shigella.Cytoskeleton (Hoboken, N.J.) · 2019Article
- Gluconeogenic growth of Vibrio cholerae is important for competing with host gut microbiota.Journal of medical microbiology · 2018Article
- Formate PromotesmBio · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Shigella flexneri, which replicates in the cytoplasm of intestinal epithelial cells, can use the Embden-Meyerhof-Parnas, Entner-Doudoroff, or pentose phosphate pathway for glycolytic carbon metabolism. To determine which of these pathways is used by intracellular S. flexneri, mutants were constructed and tested in a plaque assay for the ability to invade, replicate intracellularly, and spread to adjacent epithelial cells. Mutants blocked in the Embden-Meyerhof-Parnas pathway (pfkAB and pykAF mutants) invaded the cells but formed very small plaques. Loss of the Entner-Doudoroff pathway gene eda resulted in small plaques, but the double eda edd mutant formed normal-size plaques. This suggested that the plaque defect of the eda mutant was due to buildup of the toxic intermediate 2-keto-3-deoxy-6-phosphogluconic acid rather than a specific requirement for this pathway. Loss of the pentose phosphate pathway had no effect on plaque formation, indicating that it is not critical for intracellular S. flexneri. Supplementation of the epithelial cell culture medium with pyruvate allowed the glycolysis mutants to form larger plaques than those observed with unsupplemented medium, consistent with data from phenotypic microarrays (Biolog) indicating that pyruvate metabolism was not disrupted in these mutants. Interestingly, the wild-type S. flexneri also formed larger plaques in the presence of supplemental pyruvate or glucose, with pyruvate yielding the largest plaques. Analysis of the metabolites in the cultured cells showed increased intracellular levels of the added compound. Pyruvate increased the growth rate of S. flexneri in vitro, suggesting that it may be a preferred carbon source inside host cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.