ArticleScientific reports2014
Cell death stages in single apoptotic and necrotic cells monitored by Raman microspectroscopy.
Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers, 1 of them a synthesis that pooled it.
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Who cites it
113 citing papers in PubMed, 1 synthesis or guideline pooled it, 306 citations in OpenAlex.
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- Enhancement of antioxidant and cytotoxicity by nanoliposome formulation of pentagamavunon-6 in breast cancer cells.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Macrophage and IL-6 signaling modulate multiple myeloma progression and response to metformin and CAR-T cell therapy.Frontiers in immunology · 2026Article
- MMP release following cartilage injury leads to collagen loss in intact tissue: A computational study.PLoS computational biology · 2026Article
- Protocol for dynamic high-throughput cell death screening of primary phagocytes following microplastic and nanoplastic exposure.STAR protocols · 2025Article
- High-Resolution Imaging of Morphological Changes Associated with Apoptosis and Necrosis Using Single-Cell Full-Field Optical Coherence Tomography.Biosensors · 2025Article
- Emerging regulated cell death mechanisms in bone remodeling: decoding ferroptosis, cuproptosis, disulfidptosis, and PANoptosis as therapeutic targets for skeletal disorders.Cell death discovery · 2025Review
- Evidence of Time-Dependent Hepatic Cytotoxicity and Mitochondrial Remodelling Induced by Palmitoyl Epigallocatechin Gallate vs. Its Native (Poly)Phenol.Molecules (Basel, Switzerland) · 2025Article
- Fluorescence Guided Raman Spectroscopy enables the training of robust support vector machines for the detection of tumour marker proteins.Scientific reports · 2025Article
- Bacterioruberin extract from Haloferax mediterranei induces apoptosis and cell cycle arrest in myeloid leukaemia cell lines.Scientific reports · 2025Article
- Article
- Mapping Selenium Nanoparticles Distribution Inside Cells through Confocal Raman Microspectroscopy.ACS applied materials & interfaces · 2025Article
- Cholesterol depletion suppresses thermal necrosis resistance by alleviating an increase in membrane fluidity.Scientific reports · 2025Article
- Machine learning with label-free Raman microscopy to investigate ferroptosis in comparison with apoptosis and necroptosis.Communications biology · 2025Article
- Bisphenol Analogs Downregulate the Self-Renewal Potential of Spermatogonial Stem Cells.The world journal of men's health · 2025Article
- Mapping cellular stress and lipid dysregulation in Alzheimer-related progressive neurodegeneration using label-free Raman microscopy.Communications biology · 2024Article
- Label-Free Raman Spectroscopy for Assessing Purity and Maturation of hiPSC-Derived Cardiac Tissue.Analytical chemistry · 2024Article
53 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although apoptosis and necrosis have distinct features, the identification and discrimination of apoptotic and necrotic cell death in vitro is challenging. Immunocytological and biochemical assays represent the current gold standard for monitoring cell death pathways; however, these standard assays are invasive, render large numbers of cells and impede continuous monitoring experiments. In this study, both room temperature (RT)-induced apoptosis and heat-triggered necrosis were analyzed in individual Saos-2 and SW-1353 cells by utilizing Raman microspectroscopy. A targeted analysis of defined cell death modalities, including early and late apoptosis as well as necrosis, was facilitated based on the combination of Raman spectroscopy with fluorescence microscopy. Spectral shifts were identified in the two cell lines that reflect biochemical changes specific for either RT-induced apoptosis or heat-mediated necrosis. A supervised classification model specified apoptotic and necrotic cell death based on single cell Raman spectra. To conclude, Raman spectroscopy allows a non-invasive, continuous monitoring of cell death, which may help shedding new light on complex pathophysiological or drug-induced cell death processes.
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