Evidence map›Paper›PMID 24731858›Full record

ArticleDental materials : official publication of the Academy of Dental Materials2014

Prevascularization of biofunctional calcium phosphate cement for dental and craniofacial repairs.

Wenchuan Chen, WahWah Thein-Han, Michael D Weir, Qianming Chen, Hockin H K Xu

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Dental materials : official publication of the Academy of Dental Materials, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
6.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 58 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Regenerative Approaches for the Treatment of Large Bone Defects.Tissue engineering. Part B, Reviews · 2021
    Review
  5. Article
  6. Article
  7. Article
  8. Oxygen and nutrient delivery in tissue engineering: Approaches to graft vascularization.Journal of tissue engineering and regenerative medicine · 2019
    Review
  9. Article
  10. Regenerative biomaterials · 2019
    Article
  11. Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. A self-setting iPSMSC-alginate-calcium phosphate paste for bone tissue engineering.Dental materials : official publication of the Academy of Dental Materials · 2016
    Article
  17. Review
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Wenchuan ChenState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China; Biomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
WahWah Thein-HanBiomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
Michael D WeirBiomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
Qianming ChenState Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan 610041, China.
Hockin H K XuBiomaterials & Tissue Engineering Division, Department of Endodontics, Prosthodontics and Operative Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA; Center for Stem Cell Biology and Regenerative Medicine, University of Maryland School of Medicine, Baltimore, MD 21201, USA; University of Maryland Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, MD 21201, USA; Department of Mechanical Engineering, University of Maryland, Baltimore County, MD 21250, USA. Electronic address: hxu@umaryland.edu.
University of Maryland, Baltimore · USSichuan University · CN

Funding

Injectable and Strong Nano-Apatite/Stem Cell Scaffolds for Bone RegenerationR01DE014190 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI XU, HUAKUN · 2003 to 2013
$3.4M
Human Embryonic Stem Cells in Calcium Phosphate Constructs for Bone RegenerationR21DE022625 · NIDCR · UNIVERSITY OF MARYLAND BALTIMORE · PI XU, HUAKUN · 2012 to 2013
$413k
NIDCR NIH HHS R01 DE014190NIDCR NIH HHS R01 DE14190NIDCR NIH HHS R21 DE022625NIDCR NIH HHS R21 DE22625
6 · The paper itself

Abstract

objectivesCalcium phosphate cement (CPC) is promising for dental and craniofacial repairs. Vascularization in bone tissue engineering constructs is currently a major challenge. The objectives of this study were to investigate the prevascularization of macroporous CPC via coculturing human umbilical vein endothelial cells (HUVEC) and human osteoblasts (HOB), and determine the effect of RGD in CPC on microcapillary formation for the first time.

methodsMacroporous CPC scaffold was prepared using CPC powder, chitosan liquid and gas-foaming porogen. Chitosan was grafted with Arg-Gly-Asp (RGD) to biofunctionalize the CPC. HUVEC and HOB were cocultured on macroporous CPC-RGD and CPC control without RGD for up to 42d. The osteogenic and angiogenic differentiation, bone matrix mineral synthesis, and formation of microcapillary-like structures were measured.

resultsRGD-grafting in CPC increased the gene expressions of osteogenic and angiogenic differentiation markers than those of CPC control without RGD. Cell-synthesized bone mineral content also increased on CPC-RGD, compared to CPC control (p<0.05). Immunostaining with endothelial marker showed that the amount of microcapillary-like structures on CPC scaffolds increased with time. At 42d, the cumulative vessel length for CPC-RGD scaffold was 1.69-fold that of CPC control. SEM examination confirmed the morphology of self-assembled microcapillary-like structures on CPC scaffolds. SIGNIFICANCE: HUVEC+HOB coculture on macroporous CPC scaffold successfully achieved prevascularization. RGD incorporation in CPC enhanced osteogenic differentiation, bone mineral synthesis, and microcapillary-like structure formation. The novel prevascularized CPC-RGD constructs are promising for dental, craniofacial and orthopedic applications.

Indexed as

Calcium PhosphatesDental CementsDental Restoration, PermanentBone and BonesBone RegenerationCoculture TechniquesHumansHuman Umbilical Vein Endothelial CellsOsteoblastsTissue Engineeringcalcium phosphateCalcium PhosphatesDental CementsBone regenerationEndothelial cellsMacroporous calcium phosphate cementOsteoblastsPrevascularizationRGD biofunctionalization

Identifiers

PMID24731858
PMCPMC4321737
OpenAlexW2107487272

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.