Evidence map›Paper›PMID 24731550›Full record

ArticleInfectious agents and cancer2014

The Epstein Barr-encoded BART-6-3p microRNA affects regulation of cell growth and immuno response in Burkitt lymphoma.

Maria Raffaella Ambrosio, Mohsen Navari, Lorena Di Lisio, Eduardo Andres Leon, Anna Onnis, Sara Gazaneo, Lucia Mundo, Cristina Ulivieri, Gonzalo Gomez, Stefano Lazzi and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infectious agents and cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
4.6field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 77 citations in OpenAlex.

  1. Review
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  3. miRNA profiling in pediatric and young adult Burkitt leukemia and lymphoma.Virchows Archiv : an international journal of pathology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 2 countries.

Maria Raffaella Ambrosio *Department of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Mohsen Navari *Department of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Lorena Di LisioDepartment of Pathology, Hospital Universitario Marques de Valdecilla, IFIMAV, Santander, Spain.
Eduardo Andres LeonBioinformatics Unit (UBio), Structural Biology and Biocomputing Programme, Spanish National Cancer Research Centre (CNIO), Madrid, Spain.
Anna OnnisDepartment of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Sara GazaneoDepartment of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Lucia MundoDepartment of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Cristina UlivieriDepartment of Life Sciences, University of Siena, Siena, Italy.
Gonzalo GomezDepartment of Pathology, Hospital Universitario Marques de Valdecilla, IFIMAV, Santander, Spain.
Stefano LazziDepartment of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Miguel Angel PirisDepartment of Pathology, Hospital Universitario Marques de Valdecilla, IFIMAV, Santander, Spain.
Lorenzo LeonciniDepartment of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
Giulia De FalcoDepartment of Medical Biotechnologies, University of Siena, Via delle Scotte, 6-53100 Siena, Italy.
University of Siena · ITFundación Marques de Valdecilla · ESSpanish National Cancer Research Centre · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundBurkitt lymphoma is an aggressive B-cell lymphoma presenting in three clinical forms: endemic, sporadic and immunodeficiency-associated. More than 90% of endemic Burkitt lymphoma carry latent Epstein-Barr virus, whereas only 20% of sporadic Burkitt lymphoma are associated with Epstein-Barr infection. Although the Epstein-Barr virus is highly related with the endemic form, how and whether the virus participates in its pathogenesis remains to be fully elucidated. In particular, the virus may impair cellular gene expression by its own encoded microRNAs.

methodsUsing microRNA profiling we compared Epstein-Barr-positive and Epstein-Barr-negative Burkitt lymphoma cases for both cellular and viral microRNAs. The array results were validated by qRT-PCR, and potential targets of viral microRNAs were then searched by bioinformatic predictions, and classified in functional categories, according to the Gene Ontology. Our findings were validated by in vitro functional studies and by immunohistochemistry on a larger series of cases.

resultsWe showed that a few cellular microRNAs are differentially expressed between Epstein-Barr-positive and Epstein-Barr-negative Burkitt lymphoma cases, and identified a subset of viral microRNAs expressed in Epstein-Barr-positive Burkitt lymphomas. Of these, we characterized the effects of viral BART6-3p on regulation of cellular genes. In particular, we analyzed the IL-6 receptor genes (IL-6Rα and IL-6ST), PTEN and WT1 expression for their possible relevance to Burkitt lymphoma. By means of immunohistochemistry, we observed a down-regulation of the IL-6 receptor and PTEN specifically in Epstein-Barr-positive Burkitt lymphoma cases, which may result in the impairment of key cellular pathways and may contribute to malignant transformation. On the contrary, no differences were observed between Epstein-Barr-positive and Epstein-Barr-negative Burkitt lymphoma cases for WT1 expression.

conclusionsOur preliminary results point at an active role for the Epstein-Barr virus in Burkitt lymphomagenesis and suggest new possible mechanisms used by the virus in determining dysregulation of the host cell physiology.

Indexed as

Burkitt lymphomaEBVMicroRNAs

Identifiers

PMID24731550
PMCPMC4005456
OpenAlexW2161146599

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.