ArticleAnalytical chemistry2014
Multicolor fluorescent biosensor for multiplexed detection of DNA.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Dual Paper-Based Electrochemical Device for Multiplex Detection of Triple-Negative Breast Cancer miRNA Signatures.Analytical chemistry · 2026Article
- Rapid and quantitative detection of DNA hybridization using a simplified Fabry-Perot interferometric biosensor.RSC advances · 2024Article
- Nanotechnology-Assisted Biosensors for the Detection of Viral Nucleic Acids: An Overview.Biosensors · 2023Review
- Aluminum Oxide-Coated Particle Differentiation Employing Supervised Machine Learning and Impedance Cytometry.IEEE International Conference on Nano/Micro Engineered and Molecular Systems. IEEE International Conference on Nano/Micro Engineered and Molecular Systems · 2022Article
- Review
- A Sensitive Thrombin Aptasensor Based on Target Circulation Strategy.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Article
- Label-Free Detection ofSensors (Basel, Switzerland) · 2019Article
- Nanostructure and Corresponding Quenching Efficiency of Fluorescent DNA Probes.Materials (Basel, Switzerland) · 2018Article
- Functionalized vertical GaN micro pillar arrays with high signal-to-background ratio for detection and analysis of proteins secreted from breast tumor cells.Scientific reports · 2017Article
- Single-Step DNA Detection Assay Monitoring Dual-Color Light Scattering from Individual Metal Nanoparticle Aggregates.ACS sensors · 2017Article
- A New Two-Photon Ratiometric Fluorescent Probe for Detecting Alkaline Phosphatase in Living Cells.Molecules (Basel, Switzerland) · 2016Article
- Multitarget, quantitative nanoplasmonic electrical field-enhanced resonating device (NE2RD) for diagnostics.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Target-driven DNA association to initiate cyclic assembly of hairpins for biosensing and logic gate operation.Chemical science · 2015Article
- Highly sensitive DNA detection using cascade amplification strategy based on hybridization chain reaction and enzyme-induced metallization.Biosensors & bioelectronics · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Development of efficient methods for highly sensitive and rapid screening of specific oligonucleotide sequences is essential to the early diagnosis of serious diseases. In this work, an aggregated cationic perylene diimide (PDI) derivative was found to efficiently quench the fluorescence emission of a variety of anionic oligonucleotide-labeled fluorophores that emit at wavelengths from the visible to NIR region. This broad-spectrum quencher was then adopted to develop a multicolor biosensor via a label-free approach for multiplexed fluorescent detection of DNA. The aggregated perylene derivative exhibits a very high quenching efficiency on all ssDNA-labeled dyes associated with biosensor detection, having efficiency values of 98.3 ± 0.9%, 97 ± 1.1%, and 98.2 ± 0.6% for FAM, TAMRA, and Cy5, respectively. An exonuclease-assisted autocatalytic target recycling amplification was also integrated into the sensing system. High quenching efficiency combined with autocatalytic target recycling amplification afforded the biosensor with high sensitivity toward target DNA, resulting in a detection limit of 20 pM, which is about 50-fold lower than that of traditional unamplified homogeneous fluorescent assay methods. The quencher did not interfere with the catalytic activity of nuclease, and the biosensor could be manipulated in either preaddition or postaddition manner with similar sensitivity. Moreover, the proposed sensing system allows for simultaneous and multicolor analysis of several oligonucleotides in homogeneous solution, demonstrating its potential application in the rapid screening of multiple biotargets.
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Registered trials
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